Neurohormonal Techniques in Insects

1 Proctolin: Bioassay, Isolation, and Structure.- I. Introduction.- II. Bioassay.- III. Chemistry.- References.- 2 Adipokinetic Hormone.- I. Introduction.- II. Biological (Bioassay).- III. Chemical.- References.- 3 Bioassay of Diuretic Hormone in Rhodnius.- I. Introduction.- II. Isolation of Malpighian Tubules from Rhodnius.- References.- 4 Diuretic Hormone-Extraction and Chemical Properties.- I. Introduction.- II. Assay of Hormone Activity.- III. Isolation of Diuretic Hormone Storage Tissue.- IV. Methods of Fractionation.- V. High K+ Release of Diuretic Hormone In Vitro.- VI. Properties.- VII. Cross-Reactivity of Insect Diuretic Hormones.- VIII. Concluding Remarks.- Acknowledgements.- References.- 5 Insulin-like and Glucagon-like Hormones in Insects.- I. Introduction.- II. Preparation of Tissue Extract.- III. Purification of Extract and Heterogeneity.- IV. Biological Assay.- V. Radioimmunoassay.- VI. Immunocytochemistry.- VII. Concluding Remarks.- Acknowledgement.- References.- Note Added in Proof.- 6 Bursicon.- I. Introduction.- II. Purification of Bursicon.- III. Assays for Bursicon Activity.- References.- 7 Neurohormonal Factors Involved in the Control of Pupariation.- I. What are the Pupariation Factors?.- II. Choice of Material.- III. Breeding Technique.- IV. Staging of the Larvae for Experiments on Pupanation.- V Methods of Observing and Recording Pupariation.- VI Bioassays for the Activity of the Pupariation Factors.- VII. Materials Possessing Activity of the Pupariation Factors.- VIII Chemical Identification of the Pupariation Factors.- IX. Remarks to the Mode of Action of the Pupariation Factors.- References.- 8 Cuticle Plasticizing Factors.- I. Introduction.- II. Bioassay.- III. Chemistry.- References.- 9 Eclosion Hormones.- I. Introduction.- II. Bioassay.- III. Chemistry: Isolation and Purification.- IV. Properties.- V. Biological Activity of the Purified Hormone.- Acknowledgements.- References.- 10 Diapause Hormones.- I. Introduction.- II. Materials.- III. Bioassay.- IV. Extraction.- V. Chromatographic Separation.- VI. Selective Extraction.- VII. Chromatography on Merckogel OR.- VIII. Isolation of DH-A and DH-B.- IX. Molecular Weight 233.- X. Stability of DH in Relation to Degree of Purity.- XL Activity of the Two Species.- XII. Stability and Characters.- XIII. Infrared Spectra.- XIV. Constituents.- Acknowledgments.- References.- 11 Prothoracicotropic Hormone.- I. Introduction.- II. What is Known?-Biological.- III. Bioassay.- IV. Chemistry.- References.