Methods in Molecular Biology

1. Construction of Small-Insert and Large-Insert Metagenomic Libraries

Carola Simon and Rolf Daniel

2. Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies

Jacqueline Hollensteiner, Franziska Wemheuer, Dominik Schneider, Birgit Pfeiffer, and Bernd Wemheuer

3. Functional Metagenomics as a Tool to Tap into Natural Diversity of Valuable Biotechnological Compounds

Nancy Weiland-Br uer, Livia Saleh, and Ruth A. Schmitz

4. Functional and Sequence-Specific Screening Protocols for the Detection of Novel Antimicrobial Resistance Genes in Metagenomic DNA

Supathep Tansirichaiya, William Hutton, and Adam P. Roberts

5. Identification of PKS Gene Clusters from Metagenomic Libraries Using a Next Generation Sequencing Approach

Alinne L.R. Santana-Pereira

6. Activity-Based Screening of Metagenomic Fosmid Libraries for Hydrogen-Uptake Enzymes

Nicole Adam-Beyer and Mirjam Perner

7. Functional Metagenomics Approach for the Discovery of Novel Genes Encoding Phosphatase Activity

Genis A. Castillo Villamizar, Heiko Nacke, and Rolf Daniel

8. Isolation of Genes Encoding Carbon Metabolism Pathways from Complex Microbial Communities

Aranksha Thakor, Jiujun Cheng, and Trevor C. Charles

9. Screening Metagenomes for Algae Cell Wall Carbohydrates Degrading Hydrolases in Enrichment Cultures

Jascha F.H. Macdonald, Ines Krohn, and Wolfgang R. Streit

10. The PET-Degrading Potential of Global Metagenomes: From In Silico Mining to Active Enzymes

Jennifer Chow, Pablo P rez-Garc a, Robert F. Dierkes, Hongli Zhang, and Wolfgang R. Streit

11. High-Throughput Screening for Thermostable Polyester Hydrolases

Yannick Branson, Christoffel P. S. Badenhorst, Lara Pfaff, Carolin Buchmann, Ren Wei, and Uwe T. Bornscheuer

12. Metagenomic Screening of a Novel PET Esterase via In Vitro Expression System

Yuchen Han, Robert F. Dierkes, and Wolfgang R. Streit

13. Assigning Functions of Unknown Enzymes by High Throughput Enzyme Characterization

Patricia Molina, Laura Fernandez-Lopez, Peter N. Golyshin, and Manuel Ferrer

14. Isolation of a Host-Confined Phage Metagenome Allows the Detection of Phages Both Capable and Incapable of Plaque Formation

Ines Friedrich and Robert Hertel

15. CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis

Katharina Kohm, Veronika Theresa Lutz, Ines Friedrich, and Robert Hertel

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