Part I. Cell Line Development and Selection 1. High-Throughput Synchronization of Mammalian Cell Cultures by Spiral Microfluidic Sorter Wong Cheng Lee, Ali Asgar. S. Bhagat, and Chwee Teck Lim 2. Insect Cell Line Development Using Flp-Mediated Cassette Exchange Technology Joao Vidigal, Fabiana Fernandes, Ana S Coroadinha, Ana P Teixeira, and Paula M. Alves 3. Scalable Transient Gene Expression in Adherent Mammalian Cells Using Polyethylenimine Lukas Fliedl and Christian Kaisermayer 4. Scalable Transient Protein Expression Henry C. Chiou, Sanjay Vasu, Chao Yan Liu, Isabel Cisneros, Meredith B. Jones, and Jon Zmuda 5. DNA Profiling and Characterisation of Animal Cell Lines Glyn N. Stacey, Ed Byrne, and J. Ross Hawkins Part II. Techniques for Process Development 6. Tools for High-Throughput Process and Medium Optimization Martin Jordan and Matthieu Stettler 7. Designing Media for Animal Cell Culture - CHO Cells, The Industrial Standard Karlheinz Landauer 8. Feed Optimization in Fed-Batch Culture Ana Rita Costa, Maria Elisa Rodrigues, Mariana Henriques, Rosario Oliveira, and Joana Azeredo 9. Screening and Optimization of Chemically-Defined Media and Feeds with Integrated and Statistical Approaches Zhihua Xiao, Michelle Sabourin, Graziella Piras, and Stephen Gorfien 10. Evaluation of Solid and Porous Microcarriers for Cell Growth and Production of Recombinant Proteins Maria Elisa Rodrigues, Ana Rita Costa, Mariana Henriques, Joana Azeredo, and Rosario Oliveira 11. Microbioreactors and Scale-Down Models: Growth of CHO Cells Using the Pall Micro24 MicroReactor System Steve R.C. Warr Part III. Cell Characterisation and Analysis 12. Monitoring Cell Growth, Viability and Apoptosis Michael Butler, Maureen Spearman, and Katrin Braasch 13. Metabolic Flux Estimation in Mammalian Cell Cultures Chetan T. Goudar, Richard K. Biener, James M. Piret, and Konstantin B. Konstantinov 14. Quenching Methods for the Analysis of Intracellular Metabolites Judith Wahrheit and Elmar Heinzle 15. NMR Methods for Metabolomics of Mammalian Cell Culture Bioreactors Nelly Aranibar and Michael D. Reily 16. 1H-NMR Protocol for Exometabolome Analysis of Cultured Mammalian Cells Tiago M. Duarte, Nuno Carinhas, Ana Carina Silva, Paula M. Alves, and Ana P. Teixeira Part IV. Cultivation Techniques 17. Microfluidic Perfusion Culture Koji Hattori, Shinji Sugiura, and Toshiyuki Kanamori 18. Disposable Bioreactors for Inoculum Production and Protein Expression Regine Eibl, Christian Loffelholz, and Dieter Eibl 19. Configuration of Bioreactors Dirk E. Martens, E. J. van den End, and Mathieu Streefland 20. Process Analytical Technology (PAT) in Insect and Mammalian Cell Culture Processes - Dielectric Spectroscopy and Focused Beam Reflectance Measurement (FBRM) Damir Druzinec, Katja Weiss, Christiane Elseberg, Denise Salzig, Matthias Kraume, Ralf Portner, and Peter Czermak 21. Cell Assessment by Atline Microscopy Alexander Babitzky, Patrick Lindner, and Thomas Scheper 22. Seed Train Optimization for Cell Culture Bjorn Frahm Part V. Special Applications 23. Vaccine Production - Upstream Processing with Adherent or Suspension Cell Lines Yvonne Genzel, Jana Rodig, Erdmann Rapp, and Udo Reichl 24. Efficient Processes for Protein Expression Using Recombinant Baculovirus Particles Heike Cremer, Ingrid Bechtold, Marion Mahnke, and Rene Assenberg 25. Chromatographic Techniques in the Downstream Processing of Proteins in Biotechnology Ruth Freitag 26. Virus Separation Using Membranes Tanja A. Grein, Ronald Michalsky, and Peter Czermak
