Methods in Biotechnology

By: Charles Cunningham (Editor), Andrew J. R. Porter (Editor)

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Hardcover | 24 December 1997

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Hardcover
324 Pages

Dimensions(cm)
24.13 x 16.51 x 2.54

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Recombinant Proteins from Plants is one of the most exciting and fastest developing areas in biology. The latest molecular techniques are being applied to the exploitation of plants as novel expression systems for the p- duction and overproduction of heterologous and native proteins. Transgenic plant technology is currently used in three broad areas: the expression of - combinant proteins to improve crop quality by increasing disease/pest res- tance or increasing tolerance to stress, optimizing plant productivity and yield by the genetic manipulation of metabolic pathways, and the large-scale co- effective production of recombinant proteins for use as specialist industrial or therapeutic biomolecules. The intention of Recombinant Proteins from Plants is to provide c- prehensive and detailed protocols covering all the latest molecular approaches. Because the production oftransgenic plants has become routine in many la- ratories, coverage is also given to some of the more "classical" approaches to the separation, analysis, and characterization of recombinant proteins. The book also includes areas of research that we believe will become increasingly important in the near future: efficient transformation of monocots with Agrobacterium optimizing the stability of recombinant proteins, and a section highlighting the immunotherapeutic potential of plant-expressed proteins.

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You Can Find This Book In

Non-Fiction Science Biology, Life Sciences Botany & Plant Sciences Engineering & Technology Biochemical Engineering Biotechnology Medicine Medicine in General Cellular Biology Molecular Biology

Rice Transformation by Agrobacterium Infection
Barley as a Producer of Heterologous Protein
Introducing and Expressing Genes in Legumes
Use of the GUS Reporter
Expression of Recombinant Proteinase Inhibitors in Plants
Detection of Recombinant Viral Coat Protein in Transgenic Plants
Synthesis of Recombinant Human Cytokine GM-CSF in the Seeds of Transgenic Tobacco Plants
Tobamovirus Vectors for Expression of Recombinant Genes in Plants
Single-Chain Fv Antibodies Expressed in Plants
Characterization and Applications of Plant-Derived Recombinant Antibodies
Production of Recombinant Antibodiesin Plant Suspension Cultures
Production of Foreign Proteins in Tobacco Cell Suspension Culture
Transient Gene Expression in Plant Protoplasts
Stability of Recombinant Proteins in Plants
Trafficking and Stability of Heterologous Proteinsin Transgenic Plants
Screening for Transgenic Lines with Stableand Suitable Accumulation Levels of a Heterologous Protein
Manipulation of Photosynthetic Metabolism
Quantification of Heterologous Protein Levelsin Transgenic Plants by ELISA
Analysis of N- and O-Glycosylation of Plant Proteins A.-C. Fitchette-Lain'
Two-Dimensional Electrophoresis: A Tool for Protein Separation and Processing for Microsequencing
Index
Table of Contents provided by Publisher. All Rights Reserved.

Methods in Biotechnology

At a Glance

Hardcover

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How to return your order

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