Methods in Molecular Biology

Part I. Immunotoxin Construction and Development. Development and Activities of the BR96-Doxorubicin Immunoconjugate, Ingegerd Hellstrom, Karl Erik Hellstrom, and Peter D. Senter. CD7-Specific Single Chain Fv Immunotoxins: Design and Expression, Christopher A. Pennell and Mary E. Pauza. Recombinant Fusion Toxins Directed Against the Human Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) Receptor, Yu Shao, Berta E. Warman, and John P. Perentesis. Synthesis of Monensin Derivatives and Their Effect on the Activity of Ricin A-Chain Immunotoxins, Marco Colombatti and Franco Dosio. Purification and Conjugation of Type 1 Ribosome-Inactivating Proteins, Luigi Barbieri, Andrea Bolognesi, and Fiorenzo Stirpe. Comparison of Immunotoxins Bearing a Single Saporin Molecule with Multiple Toxin Conjugates, David J. Flavell and Sopsamorn U. Flavell. Modulation of Growth Factor Receptor Expression by Irradiation for Immunotoxin Targeting, Ki-Uk Kim, Hsaio-Tzu Ni, Stephen R. Spellman, Kwan H. Cho, Walter C. Low, and Walter A. Hall. Quantification of Immunotoxin Number for Complete Therapeutic Response, Robert J. Kreitman. In Vitro Studies of Ricin A-Chain-Induced Vascular Leak Syndrome, Alan L. Lindstrom and Christopher A. Pennell. Part II. Oncologic Applications of Immunotoxins. Immunotoxin Treatment of Brain Tumors, Walter A. Hall. Cytotoxins Directed at Interleukin-4 Receptors as Therapy for Human Brain Tumors, Raj K. Puri. Bispecific Monoclonal Antibodies for the Targeting of Type I Ribosome-Inactivating Proteins Against Hematological Malignancies, Silvano Ferrini, Sabrina Sforzini, and Silvana Canevari. Tyrosine Kinase Inhibitors Against EGF Receptor-Positive Malignancies, Elise A. Sudbeck, Sutapa Ghosh, Xing-Ping Liu, Yaguo Zheng, Dorothea E. Myers, and Fatih M. Uckun. Targeting Tumor Vasculature Using VEGF-Toxin Conjugates, S. Ramakrishnan, Robert Wild, and Dana Nojima. Gene Therapy with Immunotoxins, Daniel A. Vallera. Part III. Alternative Applications of Immunotoxins. Effects of Selective Immunotoxic Lesions on Learning and Memory, Mark G. Baxter. Targeting Toxins to Neural Antigens and Receptors, Ronald G. Wiley. In Vivo Testing of Anti-HIV Immunotoxins, Seth H. Pincus, Tamera K. Marcotte, Brent M. Forsyth, and Hua Fang. Index.