Imaging Mass Spectrometry

Protocols for Mass Microscopy

By: Mitsutoshi Setou (Editor)

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Paperback | 4 March 2010

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276 Pages

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23.5 x 15.88 x 1.27

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Addressing the widespread need for a practical guide to imaging mass spectrometry (IMS), this book presents the protocols of IMS technology. As that technology expands, research groups around the world continue its development. Pharmaceutical companies are using IMS for drug analyses to study pharmacokinetics and medical properties of drugs. Drug research and disease-related biomarker screening are experiencing greater use of this technology, with a concurrent increase in the number of researchers in academia and industry interested in wider applications of IMS. Intended for beginners or those with limited experience with IMS technology, this book provides practical details and instructions needed for immediate know-how, including the preparation of animal tissue samples, the application of a matrix, instrumental operations, and data analysis, among others. By describing the foundations of IMS, this volume contributes to the ongoing development of the field and to progress in human health.

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Innovation of Imaging Mass Spectrometry
IMS as an Historical Innovation	p. 3
Planning the Sample Preparation
Guide to Planning the Sample Preparation Step	p. 11
Sample Preparation
Animal Care and Tissue Sample Extraction for IMS	p. 33
Preparing Biological Tissue Sections for Imaging Mass Spectrometry	p. 41
Matrix Choice	p. 55
Methods of Matrix Application	p. 71
Matrix Application with ImagePrep	p. 87
Instrumental Operation for IMS
Method of Operating AXIMA-QIT as Imaging Instrument	p. 95
Analysis of Measurement Data
Ion Image Reconstruction Using BioMap Software	p. 113
Statistical Procedure for IMS Data Analysis	p. 127
Statistical Analysis of IMS Dataset with ClinproTool Software	p. 143
Applications of MALDI-IMS
Imaging of Cultured Cells by Mass Spectrometry	p. 159
TLC-Blot-MALDI-IMS	p. 169
IMS Applications Provided by Manufacturers
Applied Biosystems	p. 181
Bruker Daltonics	p. 199
Imaging and Molecular Identification of Biomolecules on Tissue Sections with AXIMA-QIT: Shimadzu Corporation	p. 209
MALDI Imaging with Ion-Mobility MS: Waters Corporation	p. 221
Comparison of SIMS and MALDI for Mass Spectrometric Imaging
Comparison of SIMS and MALDI for Mass Spectrometric Imaging	p. 235
Index	p. 259
Table of Contents provided by Ingram. All Rights Reserved.

Imaging Mass Spectrometry

Protocols for Mass Microscopy

At a Glance

Paperback

Shipping

How to return your order

Defective items

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