| Structural Analysis of Sugar Chains | |
| Release of Sugar Chains and Labeling | |
| Chemical Liberation of N-linked Oligosaccharides from Glycoproteins | p. 5 |
| Release of N-glycans by Enzymatic Methods | p. 7 |
| Release of O-glycans by Chemical Methods | p. 12 |
| Release of O-glycans by Enzymatic Methods | p. 14 |
| Ceramidase and Related Enzymes | p. 18 |
| Labeling of Oligosaccharides | p. 22 |
| Chemical Labeling of Sialyloligo/polymer | p. 24 |
| Separation of Oligosaccharides by 2D HPLC | p. 28 |
| Analysis of Oligosaccharides by Capillary Electrophoresis | p. 30 |
| Sequence Analysis | |
| Energy-Resolved Mass Spectrometry (ERMS) of Oligosaccharide | p. 37 |
| LC/MS of N-Linked Oligosaccharides | p. 39 |
| Convenient Structural Characterization of Intact Glycosphingolipids by MALDI-TOF Mass Spectrometry with Increased Laser Power and Cooling Gas Flow | p. 42 |
| Structural Analyses of Glycoconjugates by NMR | p. 45 |
| Sugar Chain Analysis by Enzymatic Digestion and 2D Mapping by HPLC | p. 51 |
| Analysis of Binding Sites of Sugar Chains by Methylation Analysis | p. 54 |
| Glycan Profiling | p. 56 |
| Monoclonal Antibody as a Clue to Structural Analysis of Bioactive Functional Glycoconjugates | p. 60 |
| Microsequencing of Functional Chondroitin Sulfate Oligosaccharides | p. 64 |
| Structural Characterization of PA-oligosaccharide Isomers Derived from Glycosphingolipids by MALDI-TOF Mass Spectrometry | p. 70 |
| Structural Analysis of Phospho-Glycosphingolipids in Lower Animals | p. 74 |
| Structural Analysis of Polysialic Acid | p. 77 |
| Milk Oligosaccharides: Structural Characterization and Future Aspects | p. 82 |
| Sugar Chain Analysis by Mass Spectrometry | |
| An XML Description of Carbohydrate Structures | p. 89 |
| Construction of a Diagnostic Library for Glycans Using Multistage Tandem Mass Spectrometry (MS") | p. 92 |
| A Method for Large-Scale Analysis for N-linked Glycoproteins by the Glycosylation Site-Specific Stable Isotope-Labeling and LC/MS Shotgun Technology | p. 94 |
| Mass Spectrometry of Glycopeptides | p. 98 |
| Development of MS Method for the Analysis of Sugar Peptide | p. 100 |
| Calculations for Saccharides by the Use of MS Data | p. 105 |
| Analysis of the Three-dimensional Structure of Sugar Chains | |
| Molecular Modeling of Oligosugar Structures | p. 109 |
| Prediction of Sugar-Binding Sites on Proteins | p. 111 |
| Analysis of Sugar-Protein Interactions | |
| Frontal Affinity Chromatography: An Effective Analytical Tool for Protein-Sugar Interaction | p. 117 |
| Analyses of Sugar-Protein Interactions by NMR | p. 121 |
| Surface Plasmon Resonance and Sugar Chip Analysis for Sugar Chain- Protein Interactions | p. 124 |
| Analysis of Interactions Between Carbohydrates and Proteins Using Capillary Affinity Electrophoresis | p. 127 |
| Equilibrium Dialysis | p. 132 |
| Interaction Assay of Oligosaccharide with Protein Using Fluorescence Polarization (FP) and Fluorescence Correlation Spectroscopy (FCS) | p. 134 |
| Development of Neoglycoconjugate Probes and Detection of Lectins | p. 138 |
| Chemical Synthesis of Sugar Chains | |
| Chemical Synthesis of Sugar Chains | |
| Synthesis of Glycolipid and Its Application | p. 147 |
| Chemo-Enzymatic Synthesis of Glycoconjugates | p. 151 |
| Construction of O-Linked Glycopeptide Library Using Human Glycosyltransferases | p. 155 |
| Alteration of N-Linked Oligosaccharides | p. 158 |
| Stationary Solid-Phase Reaction (SSPR) for Oligosaccharide Synthesis | p. 164 |
| Sugar Chain Synthesis by the Use of Cell Functions | p. 166 |
| Glyco-Chemistry Cycle System Based on Glycosidases | p. 169 |
| Endoglycosidases (Glycosaminoglycans) | p. 173 |
| Oligosaccharide Synthesis Based on Combinatorial Chemistry and Labo Automation | p. 177 |
| Endoglycosidases (Glycoproteins) | p. 182 |
| Glycosaminoglycans | p. 186 |
| Recent Advances in the Production of Mammalian-Type Sugar Chains in Yeast | p. 191 |
| Solid-Phase Synthesis of Glycopeptides | p. 195 |
| Efficient Synthesis of Oligosaccharides and Synthesis of Pathogen-Associated Molecular Patterns for Their Biofunctional Studies | p. 200 |
| Sugar Polymers (Dendrimers and Pendant-Type Linear Polymers) | p. 206 |
| Rapid Synthesis of Oligosaccharides: Resin Capture-Release Strategy | p. 210 |
| Glossary | p. 217 |
| Index | p. 249 |
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