Atomic Force Microscopy in Nanobiology

Basics for AFM. A Short Story of AFM in Biology. Protocols for Specimen and Substrate Preparation, and Data Correction Methods. Chemical Modification of AFM Probe and Coupling with Biomolecules. Single Molecule Dissection and Isolation Based on AFM Nano-Manipulations. Development of AFM Technology: Imaging Dynamics and Complexities. Structural Biology with Cryo AFM and Computational Modeling. Development of Non-Contact High-Resolution AFM and its Biological Applications. Development of Recognition Imaging - From Molecule to Cells. Development of High-Speed AFM and Its Biological Applications. Real-Time AFM Combined with an Inverted Optical Microscope for Wetcell/Tissue Imaging. Imaging Membranes, Proteins and DNA. AFM imaging of Cells (Fixed and Living) and of Particularcellular Organelles and Compartments. Determination of the Architecture of Multi-Subunit Proteins Using AFM Imaging. Capturing Membrane Proteins at Work. Enzymes and DNA: Molecular Motors in Action. Genome Folding Mechanisms in the Three Domains of Life Revealed by AFM Imaging. Imaging, Force Spectroscopy and Physiology. Membrane Dynamics: Lipid-Protein Interaction Studied by AFM. Nano Surgery and Cytoskeletal Mechanics of Single Cell. Functional Investigations on Nuclear Pores with Atomic Force Microscopy. Mechanotransduction: Probing its Mechanisms at the Nanoscale using the Atomic Force Microscope.