Part I. Cloning, Expression and Protein Production
1. DisMeta - a Meta Server for Construct Design and Optimization
Yuanpeng Janet Huang, Thomas B. Acton, and Gaetano T. Montelione
2. Stable Expression Clones and Auto-Induction for Protein Production in E. Coli
F. William Studier
3. High-Throughput Expression Screening and Purification of Recombinant Proteins in E. coli
Natalie J. Saez and Renaud Vincentelli
4. Medium Throughput Production of Recombinant Human Proteins: Ligation Independent Cloning
Claire Strain-Damerell, Nicola A. Burgess-Brown, Pravin Mahajan, and Opher Gileadi
5. Medium Throughput Production of Recombinant Human Proteins: Protein Production in E. coli
Nicola A. Burgess-Brown, Pravin Mahajan, Claire Strain-Damerell, Opher Gileadi, and Susanne Graslund
6. Medium Throughput Production of Recombinant Human Proteins: Protein Production in Insect Cells
Pravin Mahajan, Nicola A. Burgess-Brown, Claire Strain-Damerell, and Opher Gileadi
7. OmniBac: Universal Multigene Transfer Plasmids for Baculovirus Expression Vector Systems
Deepak B. Thimiri Govinda Raj, Lakshmi S. Vijayachandran, and Imre Berger
8. Multiprotein Complex Production in Insect Cells by Using Polyproteins
Yan Nie, Itxaso Bellon-Echeverria, Simon Trowitzsch, Christoph Bieniossek, and Imre Berger
9. Expression Screening in Mammalian Suspension Cells
Susan D. Chapple and Michael R. Dyson
10. Cell-free Expression of Protein Complexes for Structural Biology
Takaho Terada, Takeshi Murata, Mikako Shirouzu, and Shigeyuki Yokoyama
11. Cell-Free Protein Synthesis for Functional and Structural Studies
Shin-ichi Makino, Emily T. Beebe, John L. Markley, and Brian G. Fox
12. Insoluble Protein Purification with Sarkosyl: Facts and Precautions
Ben Chisnall, Courtney Johnson, Yavuz Kulaberoglu, and Yu Wai Chen
Part II. Experimental Structure Determination and Characterisation
13. Estimation of Crystallization Likelihood Through a Fluorimetric Thermal Stability Assay
Vincent Mariaule, Florine Dupeus, and Jose A. Marquez
14. CrystalDirect(TM) A Novel Approach for Automated Crystal Harvesting Based on Photoablation of Thin Films
Jose A. Marquez and Florent Cipriani
15. Methods to Refine Macromolecular Structures in Cases of Severe Diffraction Anisotropy
Michael R. Sawaya
16. Applications of NMR-Based PRE and EPR-Based DEER Spectroscopy to Homodimer Chain Exchange Characterization and Structure Determination
Yunhuang Yang, Theresa A. Ramelot, Shuisong Ni, Robert M. McCarrick, and Michael A. Kennedy
17. A Cost-Effective Protocol for the Parallel Production of Libraries of 13CH3-Specifically Labelled Mutants for NMR Studies of High Molecular Weight Proteins
Elodie Crublet, Rime Kerfah, Guillaume Mas, Marjolaine Noirclerc-Savoye, Violaine Lantez, Thierry Vernet, and Jerome Boisbouvier
18. High-Throughput SAXS for the Characterization of Biomolecules in Solution: A Practical Approach
Kevin N. Dyer, Michal Hammel, Robert P. Rambo, Susan E. Tsutakawa, Ivan Rodic, Scott Classen, John A. Tainer, and Greg L. Hura
19. Measuring Spatial Restraints on Native Protein Complexes using Isotope-Tagged Chemical Cross-Linking and Mass Spectrometry
Franz Herzog
Part III. Computational Methods and Structural Data Analyses
20. Modeling of Proteins and Their Assemblies with the Integrative Modeling Platform
Benjamin Webb, Ker
