Recombinant DNA Principles and Methodologies

Why Cloning?, James J. Greene Biochemistry of Nucleic Acids, Paul S. Miller Enzymology of Recombinant DNA, Frank J. Castora Basic Escherichia coli Genetics Useful for Recombinant DNA Techniques, Darren D. Sledjeski and Susan Garges Formulation of Cloning Strategies, John B. Dame Isolation and Purification of Nucleic Acids, James J. Greene Gel Electrophoresis, Southern and Northern Analysis, James J. Greene and Frank J. Castora Hybridization Probes, Frank J. Castora and James J. Greene cDNA Cloning and the Construction of Recombinant DNA, Dante S. Zarlenga Molecular Cloning by Plasmid Vectors, Paulina Balb¡s and Francisco Bol­var Phage Lambda: Basic Biology and Use as a Vector, Susan Frackman and Michael Feiss Cosmid Cloning, John Collins and Werner Lindenmaier Multipurpose Cloning Vectors, August J. Sick, Joseph M. Fernandez, and Jay M. Short Mammalian Expression Vectors, Ricky R. Hirschhorn and Nava Sarver Approaches to the Expression of Foreign Genes, Allan R. Shatzman Screening for Specific Recombinant Clones, Lucia Irene Gonz¡lez-Villase±or and Mark M. Manak Principles and Applications of the Polymerase Chain Reaction, Garbriela S. Dveksler and Carl W. Dieffenbach DNA Sequencing, Maurice A. Kashdan, Phillip T. Moen, Jr.,Venigalla B. Rao, and Bachtoi S. Rao Site-Directed Mutagenesis, Alfred Johnson and Marvin Reitz