Methods in Molecular Biology

Part I. Introduction and Overview 1. Protein-Ligand Interactions: Fundamentals Mark A. Williams 2. Protein Sample Characterization Tina Daviter and Remi Fronzes 3. Measurement of Protein-Ligand Complex Formation Peter N. Lowe, Cara Vaughan, and Tina Daviter Part II. Quantitation of Thermodynamics and Kinetics 4. Isothermal Titration Calorimetry for Studying Protein-Ligand Interactions Luminita Damian 5. Rapid Mixing Kinetic Techniques Stephen R. Martin and Maria J. Schilstra 6. Protein-Ligand Interactions Using SPR Systems Asa Frostell, Lena Vinterback and Hans Sjobom Part III. Spectroscopic Methods 7. Fluorescence Techniques in Analysis of Protein-Ligand Interactions Gabor Mocz and Justin A. Ross 8. Circular and Linear Dichroism Spectroscopy for the Study of Protein-Ligand Interactions Tina Daviter, Nikola Chmel, and Alison Rodger 9. Analyzing Protein-Ligand Interactions by Dynamic NMR Spectroscopy Anthony Mittermaier and Erick Meneses 10. Studying Metal Ion - Protein Interactions: Electronic Absorption, Circular Dichroism and Electron Paramagnetic Resonance Lilliana Quintanar and Lina Rivillas-Acevedo 11. Monitoring Protein-Ligand Interactions by Time-resolved FTIR Difference Spectroscopy Carsten Kotting and Klaus Gerwert Part IV. Ligand Discovery 12. Biophysical Methods in Drug Discovery from Small Molecule to Pharmaceutical Geoffrey Holdgate, Stefan Geschwindner, Alex Breeze, Gareth Davies, Nicola Colclough, David Temesi, and Lara Ward 13. Biophysical Screening for the Discovery of Small-Molecule Ligands Alessio Ciulli 14. Screening Protein-Small Molecule Interactions by NMR Ben Davis Part V. Molecules in Cellular Environments 15. Model Membrane Systems Heiko Keller, Remigiusz Worch, and Petra Schwille 16. Quantitative Fluorescence Co-localization to Study Protein-Receptor Complexes Shanica N. Pompey, Peter Michaely, and Katherine Luby-Phelps Part VI. Structural & Computational Methods 17. Studying Protein-Ligand Interactions Using X-Ray Crystallography Andrew P. Turnbull and Paul Emsley 18. Molecular Fields in Ligand Discovery Paul J. Gane and A. W. Edith Chan 19. Structure-Based Virtual Screening for Novel Ligands William R. Pitt, Mark D. Calmiano, Boris Kroeplien, Richard D. Taylor, James P. Turner, and Michael A. King