Protein Structures : Methods in Protein Structure and Stability Analysis

By: Eugene A Permyakov (Editor), Vladimir N Uversky (Editor)

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372 Pages

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Protein research is a frontier field in science. Proteins are widely distributed in plants and animals and are the principal constituents of the protoplasm of all cells, and consist essentially of combinations of a-amino acids in peptide linkages. Twenty different amino acids are commonly found in proteins, and serve as enzymes, structural elements, hormones, immunoglobulins, etc., and are involved throughout the body, and in photosynthesis. This book gathers new leading-edge research from throughout the world in this exciting and exploding field of research.

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Non-Fiction Science Chemistry Analytical Chemistry Spectrum Analysis Organic Chemistry Biology, Life Sciences Biochemistry Proteins

Introduction	p. 1
A multiparametric approach in analysis of protein structure and unfolding-refolding reaction	p. 3
Luminescence spectroscopy	p. 53
Intrinsic fluorescence in protein structure analysis	p. 55
Analysis of folded, partially folded, and misfolded proteins with fluorescent dyes	p. 73
Characterization of conformational ensembles of globular proteins along the folding/unfolding transition paths by means of ensemble and single molecule detection of fluorescence resonance energy transfer (FRET) measurements	p. 105
Steady-state quenching of fluorescence to study protein structure and dynamics	p. 153
Local dynamics of macromolecules by time-resolved fluorescence anisotropy	p. 187
Tryptophan phosphorescence of proteins	p. 201
Time resolved protein fluorescence in multi-tryptophan proteins	p. 237
Studies of metal binding proteins by intrinsic luminescence method	p. 257
Circular dichroism	p. 289
Aromatic side-chain contributions to protein circular dichroism	p. 291
Time-resolved circular dichroism as a structural probe of rhodopsin photolysis intermediates	p. 345
Table of Contents provided by Blackwell. All Rights Reserved.

Protein Structures : Methods in Protein Structure and Stability Analysis

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