Plant Vacuoles

Overview of Recent Developments.- Vacuoles Today and Twenty Years Ago.- Problems o Methodology in Study of Plant Vacuoles and Tonoplast Vesicles.- Methodological and Other Aspects of Intact Mature Higher Plant Cell Vacuoles.- Properties of Vacuoles as a Function of the Isolation Procedure.- Highly Purified Tonoplast Fractions by Preparative Free-Flow Electrophoresis.- Analysis of Vacuolar Polypeptides from Barley Mesophyll by Immunoblotting.- A Hyperpolarization-activated K+ current in Isolated Vacuoles of Acer pseudoplatanus.- A Microcomputer-based System for the Recording and Analysis of Fluorescence Transients from Membrane Vesicles.- Some Basic Characteristics of Plant Vacuoles in Relation with the Different Events During the Plant Life.- Benefits and Costs of Vacuolation.- The Photosynthetic Potential and Growth of Photoautotrophic Cell Suspension Cultures from Chenopodium rubrum: A case study.- Vacuolar Accumulation of Organic Acids and their Anions in CAM Plants.- Osmotic Factors affecting the Mobilisation of Sucrose from Vacuoles of Red Beet Storage Root Tissue.- Distribution of Potassium between Vacuole and Cytoplasm in Response to Potassium Deficiency.- Vacuolar pH Level is related to Photosynthesis in Isolated Mesophyll Protoplasts of Melilotus alba.- The Tonoplast State of Knowledge Difficulties and Strategies of Its Study.- Understanding Tonoplast Function: Some Emerging Problems.- Analysis of the Electrical Fluctuations in Red Beet (Beta vulgaris L. ) Vacuoles.- Purification and Properties of ?-mannosidase from Vacuolar Membranes of Yeast Saccharomyces cerevisiae.- Simultaneous Kinetic Measurements of Adenine-nucleotides in Isolated Vacuoles by HPLC Technique.- Comparison of Two Methods for the Isolation of Vacuoles in relation to Vacuolar ATPase Activity.- NBD-PC: A Tool to study Phospholipase Activity in Protoplasts.- Tonoplast Energization Relative Contribution of Different Proton Pumps to the Generation of Proton-Motive Force under the Conditions of Plant Life.- On the Existence of a Tonoplast-Bound ATPase.- A Third-category and a Fourth-category H+-phosphohydrolase at the Tonoplast.- Structure and Function of the Subunits of the Vacuolar Membrane H+-ATPase of Saccharomyces cerevisiae.- Differential Susceptibilities of Tonoplast ATPase and PPase to Irreversible Inhibition by Chaotropic Anions.- Generation of a pH-gradient across the Tonoplast of Vacuoles isolated from Green Suspension Cells of Chenopodium rubrum.- Mg,ATP-dependent H+ Transport in Vacuoles and Tonoplast Vesicles from Acer pseudoplatanus Cells.- The Effects of Anions on PPi-dependent Proton Transport in Tonoplast Vesicles from Oat Roots.- Changes of Vacuolar and Cytoplasmic pH associated with the Activation of the H+-pump in Elodea leaves.- Inhibition of ATP-dependent Proton Pumping in Plant Tonoplast- and Plasmalemma-enriched Fractions by Mycochromone.- Different Types of Solute Transport across the Tonoplast.- Transport of Inorganic Ions in Tonoplast Vesicles.- Wash-activated Na+/H+ Exchange in Tonoplast Vesicles from Beta vulgaris Storage Root Disks.- Isolation of Tonoplast Vesicles from Kiwi fruit cells: Electrochemical Investigation of K+ and H+ membrane Transport linked to a Mg-ATP Hydrolytic Activity.- Some Characteristics of Tonoplast NO3-transport Processes on Acer pseudoplatanus L. Cells.- Active Transport of Amino-acids and Calcium Ions in Fungal Vacuoles.- ATP-dependent Uptake of Malate in Vacuoles from CAM Plant, Kalanchoe daigremontiana.- Proton-motive Force and H+/Amino-acid Antiport at the Tonoplast of Riccia fluitans rhizoid cells, revealed by direct probing with the pH-sensitive microelectrode.- Sugar Transport across the Tonoplast of Vacuoles Isolated from Protoplasts of Jerusalem artichoke Tubers.- Mg2+-ATP-dependent Glucose Transport in Tonoplast Vesicles of Normal and Agrobacterium tumefaciens-transformed tobacco cells.- Regulation of the UDP-glucose Group Translocator by some Cytoplasmic Components.- Preliminary Evidence for Solubilization of an Active Tonoplast-bound Vectorial Sucrose Synthesizing Enzyme Complex.- Specific Uptake of the N-oxides of Pyrrolizidine Alkaloids by Cells, Protoplasts and Vacuoles from Senecio Cell Cultures.- Accumulation and Sequestration of Vacuolar Solutes.- Accumulation of Organic Solutes in Plant Vacuoles: The Interpretation of Data is not so easy.- Vacuole Function in Neurospora.- $$Delta overline mu {{}_{{{{H}^{ + }}}}}$$-controlled Reversible Fluxes of H+ and Calcium at the tonoplast but Quasi-total Citrate Sequestration within the Intact Vacuoles from the Latex Cells of Hevea brasiliensis. Implications in the Production of Natural Rubber.- Ajmalicine Transport into Vacuoles Isolated from Catharathus roseus Cells.- Role of the Vacuole in Metal Ion Trapping as Studied by in vivo31P-NMR Spectroscopy.- Plant Cell Metabolism and Vacuolar Compartmentation.- Dynamics of Lysosomal Functions in Plant Vacuoles.- Protein Degradation in Vacuoles from Acer pseudoplatanus L. Cells.- Protease Activity in Oat Leaf Vacuoles.- Compartmentation of Glycosidases in a Light Vacuole Fraction from the Latex of Lactuca sativa L..- The possible Role of Endoplasmic Reticulum in the Biosynthesis and Transport of Anthocyanin Pigments.- Patch-clamp Studies on the Transport of Ions across the Membrane of Barley Vacuoles.- Intracellular Distribution of Organic and Inorganic Anions in Mesophyll Cells : Transport Mechanisms in the Tonoplast.- Vacuolar and Cytosolic Metabolite Pools by Comparative Fractionation of Vacuolate and Evacuolate Protoplasts.- Subcellular Localization of Primary and Second Cardiac Glycosides, and the related 16?-O-glucosyl-transferase in Cell Suspension Cultures of Digitalis lanata.- The Participation of Vacuoles in Transduction of Signals Regulating the Cytoplasmic Metabolism.- Tonoplast: the Controlling Site for the in vitro Transport in Developping Soybean Cotyledons.- The Vacuole in relation to Plant Growth Regulators.- Involvement of Vacuoles in Ethylene Metabolism in Plant Cells.- The Vacuole : Possible Role in Signal Transduction versus Cytoplasmic Homeostasis.- Transtonoplast pH Changes as a Signal for Activation of the Cytosolic Metabolism in Hevea Latex Cells in Response to Treatments with Ethrel (an Ethylene Releaser). Implications for the Production of a Secondary Metabolite: Naturel Rubber.- Production of Biochemicals (Secondary Metabolites and Enzymes Sequesterated in Vacuoles) by Plant Cell Cultures.- Production of Plant Secondary Metabolites by Plant Cell Cultures in relation to the Site and Mechanism of their Accumulation.- Production of Secondary Metabolites in Cell Cultures of some Terpenoid-indole Alkaloids producing Plants.- Continuous Production of Indole-alkaloids by Gel-entrapped Cells of Catharanthus roseus.- Factors affecting Alkaloid Production in Plant Cell Cultures.- Papaver latex and Alkaloid Storage Vacuoles.- Influence of External pH on Alkaloid Production and Excretion.- Stress Metabolism in Plant Cell Cultures: Enhancement of Primary and Secondary Metabolites in Osmotically and Salt-adapted Cultures of Catharanthus roseus.- Significant Increase in Pyrimidinic Alkaloid (trigonelline) caused by Ionizing Radiation.- Participants.