Methods in Molecular Biology

By: James M. Cregg (Editor)

Write A Review

Hardcover | 8 August 2007 | Edition Number 2

At a Glance

Hardcover
284 Pages

Edition Type
Revised

Dimensions(cm)
23.5 x 15.24 x 2.54

Hardcover

$169.75

or 4 interest-free payments of $42.44 with

or

Ships in 5 to 7 business days

Pichia Protocols focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI).

This volume fully updates and expands upon the first edition of Pichia Protocols, and focuses primarily on information that has come to light since its original publication. Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system.

Industry Reviews

From the reviews of the second edition:

"P. pastoris has many superior traits. ... can grow to very high cell densities prior to switching on an inducible promoter (the AOX1 gene) for maximal recombinant protein production. The diversity of such proteins produced by expression strains of P. pastoris is quite amazing and this book describes detailed experimental protocols for a few of them (e.g. hookworm protein, botulinum toxin). ... This is a stand-alone book ... . a book that will prove valuable to yeast molecular geneticists and biotechnologists." (Graeme Walker, Microbiology Today, May, 2008)

Shipping

	Standard Shipping	Express Shipping
Metro postcodes:	$9.99	$14.95
Regional postcodes:	$9.99	$14.95
Rural postcodes:	$9.99	$14.95

Orders over $79.00 qualify for free shipping.

How to return your order

At Booktopia, we offer hassle-free returns in accordance with our returns policy. If you wish to return an item, please get in touch with Booktopia Customer Care.

Additional postage charges may be applicable.

Defective items

If there is a problem with any of the items received for your order then the Booktopia Customer Care team is ready to assist you.

For more info please visit our Help Centre.

More in Biophysics

The Body Electric : Electromagnetism and the Foundation of Life - Gary Selden

$73.75

36%
OFF

An Introduction to Conservation

3rd Edition

Paperback

RRP $170.95

$138.75

19%
OFF

Pull

how gravity shapes your body, steadies the mind, and guides our health

Paperback

RRP $36.99

$29.75

20%
OFF

Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology

8th edition

Paperback

RRP $91.95

$77.99

15%
OFF

Physics in Nuclear Medicine : With CD-ROM Expert Consult- Online and Print, 4e - James A., PhD Sorenson

$345.99

12%
OFF

Handbook of MRI Pulse Sequences

Hardcover

RRP $350.95

$274.99

22%
OFF

Bioprocess Engineering Principles

Paperback

RRP $204.95

$138.75

32%
OFF

Implantable Electronic Medical Devices

Paperback

RRP $218.95

$188.75

14%
OFF

Imaging for Students

Paperback

RRP $88.99

$69.99

21%
OFF

Introduction to Cell Mechanics and Mechanobiology - Christopher R. Jacobs

$91.75

Biophysical Models of Radiation Action : Series in Medical Physics and Biomedical Engineering - Hooshang Nikjoo

$73.99

10%
OFF

The Physics and Technology of Diagnostic Ultrasound : A Practitioner's Guide (Second Edition) - Robert Wyatt Gill

$131.75

30%
OFF

$227.75

27%
OFF

Preface	p. v
Contributors	p. ix
Introduction: Distinction Between Pichia pastoris and Other Expression Systems	p. 1
Vectors and Strains for Expression	p. 11
DNA-Mediated Transformation	p. 27
Rational Design and Optimization of Fed-Batch and Continuous Fermentations	p. 43
Saturation of the Secretory Pathway by Overexpression of a Hookworm (Necator americanus) Protein (Na-ASP1)	p. 65
Purification of the N- and C-Terminal Subdomains of Recombinant Heavy Chain Fragment C of Botulinum Neurotoxin Serotype C	p. 77
Rapid Screening of Chromatography Resins for the Purification of Proteins	p. 99
Characterization of O-Linked Saccharides on Glycoproteins	p. 107
Modification of the N-Glycosylation Pathway to Produce Homogeneous Human-Like Glycans Using GlycoSwitch Plasmids	p. 119
N-Linked Glycan Characterization of Heterologous Proteins	p. 139
Heavy Labeling of Recombinant Proteins	p. 151
Selenomethionine Labeling of Recombinant Proteins	p. 165
Selective Isotopic Labeling of Recombinant Proteins Using Amino Acid Auxotroph Strains	p. 175
Classical Genetics	p. 189
Identification of Pexophagy Genes by Restriction Enzyme-Mediated Integration	p. 203
Characterization of Protein-Protein Interactions: Application to the Understanding of Peroxisome Biogenesis	p. 219
Localization of Proteins and Organelles Using Fluorescence Microscopy	p. 239
Fluorescence Microscopy and Thin-Section Electron Microscopy	p. 251
Index	p. 261
Table of Contents provided by Ingram. All Rights Reserved.

Methods in Molecular Biology

At a Glance

Hardcover

Industry Reviews

Shipping

How to return your order

Defective items

You Can Find This Book In

Other Editions and Formats

Paperback

More in Biophysics

The Body Electric

Electromagnetism and the Foundation of Life

Introduction to Biological Physics for the Health and Life Sciences

2nd Edition

An Introduction to Conservation

3rd Edition

Pull

how gravity shapes your body, steadies the mind, and guides our health

Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology

8th edition

Physics in Nuclear Medicine

With CD-ROM Expert Consult- Online and Print, 4e

Structural Study of Amyloid-×ý

Methods in Enzymology

Handbook of MRI Pulse Sequences

Bioprocess Engineering Principles

Implantable Electronic Medical Devices

Imaging for Students

Introduction to Cell Mechanics and Mechanobiology

Introduction to Cell Mechanics and Mechanobiology

Astrobiology and the People Who Study It

Stories of Science and Self

Astrobiology and the People Who Study It

Stories of Science and Self

Biophysical Models of Radiation Action

Series in Medical Physics and Biomedical Engineering

An Introduction to Systems Biology

Design Principles of Biological Circuits

Meaning of Life and the Universe

Transforming

The Physics and Technology of Diagnostic Ultrasound

A Practitioner's Guide (Second Edition)

Movement System Variability

Pull

How Gravity Shapes Your Body, Steadies the Mind, and Guides Our Health

Polarization-Based Optical Imaging for Clinical Diagnostics

Fundamentals of Biomechanics

Recent Advances in Targeting Inflammation

Ion channels, GPCR Signaling Tools, Therapies, and Beyond

This product is categorised by