Patch-Clamp Methods and Protocols

Table of contents Part 1. Pharmacology 1. Pharmacological analysis of recombinant NR1a/2A and NR1a/2B NMDA receptors using whole-cell patch clamp method. Laszlo Fodor and Jozsef Nagy 2. Memantine as an example of a fast, voltage-dependent, open channel N-methyl-D-aspartate (NMDA) receptor blocker. Chris G. Parsons and Kate Gilling 3. Methods for evaluation of positive allosteric modulators of glutamate AMPA receptors. Edward R. Siuda, Jennifer C. Quirk and Eric S. Nisenbaum 4. Automated voltage clamp technique. Andrea Ghetti, Antonio Guia and Jia Xu 5. Flip-the-Tip: automated patch clamping based on glass electrodes. Michael Fejtl, Uwe Czubayko, Alexander Hummer, Tobias Krauter and Albrecht Lepple-Wienhues 6. The Roboocyte: Automated Electrophysiology Based on Xenopus Oocytes. Christine Leisgen, Mike Kuester and Christoph Methfessel Part 2. Physiology 7. Infrared guided laser stimulation as a tool for elucidating the synaptic site of expression of long-term synaptic plasticity. Gerhard Rammes, Mathias Eder, Walter Zieglgansberger and Hans-Ulrich Dodt 8. Single-cell RT-PCR, a Technique to Decipher the Electrical, Anatomical and Genetic Determinants of Neuronal Diversity. Maria Toledo-Rodriguez and Henry Markram 9. Mechanosensitive Ion Channels investigated simultaneously by Scanning Probe Microscopy and Patch clamp. Matthias G. Langer 10. Synaptic Connectivity in Engineered Neuronal Networks. Peter Molnar, Jung-Fong Kang, Neelima Bhargava, Mainak Das and James J. Hickman 11. Modeling of Action Potential Generation in NG108-15 Cells. Peter Molnar and James J. Hickman 12. Whole-cell Voltage-clamp on Skeletal Muscle Fibers with the Silicone-Clamp Technique. Sandrine Pouvreau, Claude Collet, Bruno Allard and Vincent Jacquemond 13. Determination of channel properties at the unitary level in adult mammalian isolated cardiomyocytes. Romain Guinamard 14. Electrophysiological Properties of Embryonic Stem Cells During Differentiation into Cardiomyocyte-like Cell Types. Antoni C.G. van Ginneken and Arnoud. C. Fijnvandraat 15. Hybrid Neuronal Network Studies Under Dynamic Clamp. Alan D. Dorval II, Jonathan Bettencourt, Theoden I. Netoff and John A. White 16. Cardiac channelopathies studied with the dynamic action potential clamp technique. Geza Berecki, Jan G. Zegers, Ronald Wilders and Antoni C.G. van Ginneken Part 3. Biophysics 17. Principles of Single-Channel Kinetic Analysis. Feng Qin * Theoretical chapter 18. Use of Xenopus oocytes to measure ionic selectivity of pore-forming peptides and ion channels. Thierry Cens and Pierre Charnet 19. Estimation of quantal parameters with multiple-probability fluctuation analysis. Chiara Saviane and Robin Angus Silver