Nitrogen Fixation

Part I: Nitrogen Fixation 1. Historic Overview of Nitrogenase Research Yilin Hu and Markus W. Ribbe 2. Mechanism of Mo-Dependent Nitrogenase Zhi-Yong Yang, Karamatullah Danyal, and Lance C. Seefeldt 3. Assembly of Nitrogenase MoFe Protein Chi-Chung Lee, Aaron W. Fay, Jared A. Wiig, Markus W. Ribbe, and Yilin Hu 4. Genomic Analysis of Nitrogen Fixation Ina P. O'Carroll and Patricia C. Dos Santos 5. Enzymatic Systems with Homology to Nitrogenase Markus J. Brocker and Jurgen Moser Part II: Genetic and Biochemical Methods 6. Molecular Biology and Genetic Engineering in Nitrogen Fixation Patricia Dos Santos 7. Purification of Nitrogenase Proteins Jared A. Wiig, Chi-Chung Lee, Aaron W. Fay, Yilin Hu, and Markus W. Ribbe 8. Assays of Nitrogenase Reaction Products William E. Newton and Michael J. Dilworth 9. Methods for Nitrogenase-Like Dark Operative Protochlorophyllide Oxidoreductase Markus J. Brocker and Jurgen Moser Part III: Spectroscopic Methods 10. X-Ray Crystallography Lauren E. Roth and F. Akif Tezcan 11. X-Ray Absorption Spectroscopy Serena DeBeer 12. Small Angle X-Ray Scattering Spectroscopy David W. Mulder and John W. Peters 13. Electron Paramagnetic Resonance Spectroscopy Karamatullah Danyal, Zhi-Yong Yang, and Lance C. Seefeldt 14. Magnetic Circular Dichroism Spectroscopy Brian J. Hales 15. Mossbauer Spectroscopy Boi Hanh Huynh Part IV: Chemical Methods 16. Protocols for Cofactor Isoation of Nitrogenase Aaron W. Fay, Chi-Chung Lee, Jared A. Wiig, Yilin Hu, and Markus W. Ribbe 17. Techniques for Functional and Structural Modeling of Nitrogenase Patrick L. Holland Part V: Theoretical Calculations 18. Nitrogenase Structure and Function Relationships by Density Functional Theory Travis V. Harris and Robert K. Szilagyi 19. Modeling the MoFe Nitrogenase System with Broken Symmetry Density Functional Theory Gregory M. Sandala and Louis Noodleman