MicroRNAs

From Basic Science to Disease Biology

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Paperback | 27 August 2009

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580 Pages

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MicroRNAs (miRNAs) are RNA molecules, conserved by evolution, that regulate gene expressions and their recent discovery is revolutionising both basic biomedical research and drug discovery. Expression levels of MiRNAs have been found to vary between tissues and with developmental stages and hence evaluation of the global expression of miRNAs potentially provides opportunities to identify regulatory points for many different biological processes. This wide-ranging reference work, written by leading experts from both academia and industry, will be an invaluable resource for all those wishing to use miRNA techniques in their own research, from graduate students, post-docs and researchers in academia to those working in R&D in biotechnology and pharmaceutical companies who need to understand this emerging technology. From the discovery of miRNAs and their functions to their detection and role in disease biology, this volume uniquely integrates the basic science with industry application towards drug validation, diagnostic and therapeutic development.

Forewords by:
Sidney Altman, Yale University, Winner of the Nobel Prize in Chemistry, 1989
and
Victor R. Ambros, Dartmouth Medical School, Co-discoverer of MicroRNAs

Industry Reviews

'... comprehensive and detailed ... compiled logically ... informative and easy to read.' Microbiology Today
'... a useful reference guide for those interested in miRNA ...' Immunology News

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Foreword
Foreword
Introduction
Discovery of MicroRNAs in Various Organisms:
The microRNAs of C. elegans
Non-coding RNAs - development of man-made vector-based intronic microRNAs
Seeing is believing: strategies for studying microRNA expression
MicroRNAs in limb development
Identification of miRNAs in plant oryza sativa
MicroRNA Functions and RNAi-mediated pathways:
Inhibition of translation initiation by a microRNA
In situ analysis of microRNA expression during vertebrate development
MicroRNA function in the nervous system
Micro RNA expression that controls the amount of branched chain Ä±-ketoacid dehydrogenase in mitochondria of human cells
MicroRNAs and the regulation of leaf shape
Computational Biology of microRNAs:
miRBase: a database of microRNA sequences, targets and nomenclature
Computational prediction of microRNA targets in vertebrates, fruitflies and nematodes
Computational approaches to elucidate miRNA biology
The RNA hybrid approach to microRNA target prediction
Machine learning predicts microRNA target sites
Models of microRNA-target coordination
Detection and Quantitation of MicroRNAs:
Detection and analysis of microRNAs using LNA (locked nucleic acid)-modified probes
Detection and quantitation of microRNAs using the RNA Invaderé“ assay
A single molecule method to quantify miRNA gene expression
Real-time quantification of MicroRNAs by TaqMané“ assays
Real-time quantification of miRNAs and mRNAs employing universal reverse transcription
MicroRNAs in Disease Biology:
Dysregulation of microRNAs in human malignancy
High throughput microRNAs profiling in cancers
Roles of microRNAs in cancer and development
miR-122 in mammalian liver
MiRNAs in glioblastoma
Role of microRNA pathway in Fragile X mental retardation
Insertion of miRNA125b-1 into immunoglobulin heavy chain gene locus mediated by V(D)J recombination in precursor B cell acute lymphoblastic leukemia
miRNAs in TPA-induced differentiation of HL-60 cells
MiRNAs in skeletal muscle differentiation
Identification and potential function of viral microRNAs
Lost in translation: regulation of HIV-1 by microRNAs and a key enzyme of RNA-directed RNA polymerase
MicroRNAs in Stem Cell Development:
MicroRNAs in the stem cells of the mouse Blastocyst
The role of miRNA in hematopoiesis
MicroRNAs in embryonic stem cell differentiation and prediction of their targets
Generation of single cell microRNA expression profile
Table of Contents provided by Publisher. All Rights Reserved.

MicroRNAs

From Basic Science to Disease Biology

At a Glance

Paperback

Industry Reviews

Shipping

How to return your order

Defective items

You Can Find This Book In

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