Methods in Molecular Biology

1. General Review on In Vitro Hepatocyte Models and their Applications Christiane Guguen-Guillouzo and Andre Guillouzo 2. Human Foetal Hepatocytes: Isolation, Characterization, and Transplantation Anne Weber, Thomas Touboul, Sylvie Mainot, and Dominique Mahieu-Caputo 3. Isolation and Culture of Primary Hepatocytes from Resected Human Liver Tissue Edward L. LeCluyse and Eliane Alexandre 4. Optimization of the Cryopreservation of Primary Hepatocytes Nicola J. Hewitt 5. Cryopreservation of Human Hepatocytes for Clinical Use Ragai R. Mitry, Sharon C. Lehec, and Robin D. Hughes 6. Hepatocyte Differentiation Katy M. Olsavsky Goyak, Elizabeth M. Laurenzana, and Curtis J. Omiecinski 7. Reversible Manipulation of Apoptosis Sensitivity in Cultured Hepatocytes by Matrix Mediated Manipulation of Signaling Activities Patricio Godoy, Markus Schug, Alexander Bauer, and Jan G. Hengstler 8. Markers and Signaling Factors for Stem Cell Differentiation to Hepatocytes: Lessons from Developmental Studies Frederic Lemaigre 9. Hepatic Stem Cells Robert E. Schwartz and Catherine Verfaillie 10. Hepatic Stem Cells and Liver Development Nalu Navarro-Alvarez, Alejandro Soto-Gutierrez, and Naoya Kobayashi 11. Generation of Hepatocytes from Human Embryonic Stem Cells Neta Lavon 12. Isolation and Culture of Adult Human Liver Progenitor Cells: In vitro Differentiation to Hepatocyte-Like Cells Sabine Gerbal-Chaloin, Cedric Duret, Edith Raulet, Francis Navarro, Pierre Blanc, Jeanne Ramos, Patrick Maurel, and Martine Daujat-Chavanieu 13. The HepaRG Cell Line: Biological Properties and Relevance as a Tool for Cell Biology, Drug Metabolism, and Virology Studies Marie-Jeanne Marion, Olivier Hantz, and David Durantel 14. Transdifferentiation of Pancreatic Cells to Hepatocytes Chia-Ning Shen and David Tosh 15. Evaluation of Drug Metabolism, Drug-Drug Interactions, and In vitro Hepatotoxicity with Cryopreserved Human Hepatocytes Albert P. Li 16. The Use of Human Hepatocytes to Investigate Drug Metabolism and CYP Enzyme Induction Sylvie Klieber, Francois Torreilles, Francois Guillou, and Gerard Fabre 17. The Use of Hepatocytes to Investigate UDP-Glucuronosyltransferases and Sulfotransferases Sylvie Fournel-Gigleux, Michael W.H. Coughtrie, Mohamed Ouzzine, and Jacques Magdalou 18. The Use of Hepatocytes to Investigate Drug Uptake Transporters Kazuya Maeda and Yuichi Sugiyama 19. Metabonomic Studies on Human Hepatocyte in Primary Culture Vincent Croixmarie, Thierry Umbdenstock, Olivier Cloarec, Amelie Moreau, Jean-Marc Pascussi, Yannick Parmentier, Claire Boursier-Neyret, and Bernard Walther 20. The Application of HepRG Cells in Evaluation of Cytochrome P450 Induction Properties of Drug Compounds Tommy B. Andersson 21. The Use of Hepatocytes to Investigate Drug Toxicity Maria Jose Gomez-Lechon, Jose V. Castell, and Maria Teresa Donato 22. The Use of Human Hepatocytes to Investigate Bile Acid Synthesis Ewa C. S. Ellis and Lisa-Mari Nilsson 23. The Use of Primary Human Hepatocytes to Investigate Blood Coagulation Factor Secretion and Activity Christine Biron-Andreani, Edith Raulet, Lydiane Pichard-Garcia, and Patrick Maurel 24. The Use of Human Hepatocytes to Investigate HCV Infection Lydiane Pichard-Garcia, Philippe Briolotti, Dominique Larrey, Antonio Sa-Cunha, Bertrand Suc, Sylvain Laporte, and Patrick Maurel 25. The Use of Hepatocytes to Investigate HDV Infection: The HDV/HepaRG Model Camille Sureau 26. Rodent Models of Liver Repopulation Helene Gilgenkrantz 27. Chimeric Mice with Humanized Liver: Tools for the Study of Drug Metabolism, Excretion, and Toxicity Stephen C. Strom, Julio Davila, and Markus Grompe 28. Bioartificial Liver Support Systems Gesine Pless 29. Human Hepatocyte Transplantation Anil Dhawan, Stephen Strom, Etienne Sokal, and Ira J. Fox