Lipid Signaling Protocols

1. Method for Assaying the Lipid Kinase Phosphatidylinositol-5-phosphate 4-kinase α in Quantitative High-Throughput Screening (qHTS) Bioluminescent FormatMindy I. Davis, Atsuo T. Sasaki, and Anton Simeonov 2. Assaying Ceramide Synthase Activity In Vitro and in Living Cells using Liquid Chromatography-Mass SpectrometryXin Ying Lim, Russell Pickford, and Anthony S Don 3. Fluorescent Assays for Ceramide Synthase ActivityTimothy A Couttas and Anthony S Don 4. Identification of the Interactome of a Palmitoylated Membrane Protein, Phosphatidylinositol 4-kinase type II AlphaAvanti Gokhale, Pearl V. Ryder, Stephanie A. Zlatic, and Victor Faundez 5. Measurement of Long Chain Fatty Acyl-CoA synthetase ActivityJoachim Fullekrug and Margarete Poppelreuther 6. Qualitative and Quantitative in vitro Analysis of Phosphatidylinositol Phosphatase Substrate SpecificityLaura Ren Huey Ip and Christina Anja Gewinner 7. Luciferase Reporter Assays to Assess Liver X Receptor Transcriptional ActivityMatthew C. Gage, Benoit Pourcet, and Ines Pineda-Torra 8. Metabolically Biotinylated Reporters for Electron Microscopic Imaging of Cytoplasmic Membrane MicrodomainsKimberly J. Krager and John G. Koland 9. Fluorescence Recovery after Photobleaching Analysis of the Diffusional Mobility of Plasma Membrane Proteins: HER3 Mobility in Breast Cancer Cell MembranesMitul Sarkar and John G. Koland 10. Isolation and Analysis of Detergent-Resistant Membrane FractionsMassimo Aureli, Sara Grassi, Sandro Sonnino, and Alessandro Prinetti 11. Detection of Isolated Mitochondria-Associated ER Membranes using the Sigma-1 ReceptorAbasha Lewis and Tsung-Ping Su 12. Using Surface Plasmon Resonance to Quantitatively Assess Lipid-Protein InteractionsKathryn Del Vecchio and Robert V. Stahelin 13. Analyzing Protein-phosphoinositide Interactions with Liposome Flotation AssaysRicarda A. Busse, Andreea Scacioc, Amanda M. Schalk, Roswitha Krick, Michael Thumm, and Karin Kuhnel 14. High-Throughput Flurometric Assay for Membrane-Protein InteractionWonhwa Cho, Hyunjin Kim, and Yusi Hu 15. Guidelines for the use of Protein Domains in Acidic Phospholipid Imaging Matthieu Pierre Platre and Yvon Jaillais 16. Analysis of Sphingolipid Synthesis and Transport by Metabolic Labeling of Cultured Cells with [3H]serine Neale D. Ridgway 17. Determination and Characterization of Tetraspanin-Associated Phosphoinositide-4 Kinases in Primary and Neoplastic Liver Cells Krista Rombouts and Vinicio Carloni 18. Analysis of the Phosphoinositide Composition of Subcellular Membrane FractionsDeborah A. Sarkes and Lucia E. Rameh 19. Single-molecule Imaging of Signal Transduction via GPI-anchored ReceptorsKenichi G. N. Suzuki 20. Measuring Phosphatidylinositol Generation on Biological MembranesMark G. Waugh 21. Assay for CDP-diacylglycerol Generation by CDS in Membrane FractionsMark G. Waugh