Lipid Signaling Protocols

1. Method for Assaying the Lipid Kinase Phosphatidylinositol-5-phosphate 4-kinase alpha in Quantitative High-Throughput Screening (qHTS) Bioluminescent Format Mindy I. Davis, Atsuo T. Sasaki, and Anton Simeonov 2. Assaying Ceramide Synthase Activity In Vitro and in Living Cells using Liquid Chromatography-Mass Spectrometry Xin Ying Lim, Russell Pickford, and Anthony S Don 3. Fluorescent Assays for Ceramide Synthase Activity Timothy A Couttas and Anthony S Don 4. Identification of the Interactome of a Palmitoylated Membrane Protein, Phosphatidylinositol 4-kinase type II Alpha Avanti Gokhale, Pearl V. Ryder, Stephanie A. Zlatic, and Victor Faundez 5. Measurement of Long Chain Fatty Acyl-CoA synthetase Activity Joachim Fullekrug and Margarete Poppelreuther 6. Qualitative and Quantitative in vitro Analysis of Phosphatidylinositol Phosphatase Substrate Specificity Laura Ren Huey Ip and Christina Anja Gewinner 7. Luciferase Reporter Assays to Assess Liver X Receptor Transcriptional Activity Matthew C. Gage, Benoit Pourcet, and Ines Pineda-Torra 8. Metabolically Biotinylated Reporters for Electron Microscopic Imaging of Cytoplasmic Membrane Microdomains Kimberly J. Krager and John G. Koland 9. Fluorescence Recovery after Photobleaching Analysis of the Diffusional Mobility of Plasma Membrane Proteins: HER3 Mobility in Breast Cancer Cell Membranes Mitul Sarkar and John G. Koland 10. Isolation and Analysis of Detergent-Resistant Membrane Fractions Massimo Aureli, Sara Grassi, Sandro Sonnino, and Alessandro Prinetti 11. Detection of Isolated Mitochondria-Associated ER Membranes using the Sigma-1 Receptor Abasha Lewis and Tsung-Ping Su 12. Using Surface Plasmon Resonance to Quantitatively Assess Lipid-Protein Interactions Kathryn Del Vecchio and Robert V. Stahelin 13. Analyzing Protein-phosphoinositide Interactions with Liposome Flotation Assays Ricarda A. Busse, Andreea Scacioc, Amanda M. Schalk, Roswitha Krick, Michael Thumm, and Karin Kuhnel 14. High-Throughput Flurometric Assay for Membrane-Protein Interaction Wonhwa Cho, Hyunjin Kim, and Yusi Hu 15. Guidelines for the use of Protein Domains in Acidic Phospholipid Imaging Matthieu Pierre Platre and Yvon Jaillais 16. Analysis of Sphingolipid Synthesis and Transport by Metabolic Labeling of Cultured Cells with [3H]serine Neale D. Ridgway 17. Determination and Characterization of Tetraspanin-Associated Phosphoinositide-4 Kinases in Primary and Neoplastic Liver Cells Krista Rombouts and Vinicio Carloni 18. Analysis of the Phosphoinositide Composition of Subcellular Membrane Fractions Deborah A. Sarkes and Lucia E. Rameh 19. Single-molecule Imaging of Signal Transduction via GPI-anchored Receptors Kenichi G. N. Suzuki 20. Measuring Phosphatidylinositol Generation on Biological Membranes Mark G. Waugh 21. Assay for CDP-diacylglycerol Generation by CDS in Membrane Fractions Mark G. Waugh