Methods in Cell Wall Cytochemistry

Preface Cell Wall-Morphology & Chemical Composition Carbohydrates Cellulose, Callose and other 1, 3-b-glucans, Chitins, Pectins, Hemicelluloses, Alginic Acid, Sulphated Polysaccharides Glycoproteins Structural Proteins-Lectins, Arabinogalacton Rich Proteins (AGP's), Hydroxy Proline Rich Proteins, Proline Rich Proteins, Glycine Rich Proteins, Threonine Rich Proteins, Thionins, Pistil Specific Extensin-Like Proteins Enzymes-Peroxidase, Polyamineoxidase, Phosphatase, Protease, Nitrate Reductase, Malate Dehydrogenase, Proline Hydroxylase, Glycosylhydrolases and Transferases, Non-Specific Esterases, and Ribonucleuses Lipids and Related Substances-Cutin, Suberin, Waxes, Sporopollenin, Pollenkitt, and Tryphine Lignin and Other Phenolic Substances-Lignins, Phenolic Acids and Amides Mineral Substances-Silica, Calcium, and Boron Light Microscopic Cytochemistry Introduction Tissue Preparation-Use of Fresh Living Tissue, Use of Frozen Tissue, Freeze Substitution and Drying, Chemical Fixation, Dehydration and Clearing, Infiltration and Embedding, Sectioning of Materials Stains and Staining-Introduction, Control Procedures, Incubation of Materials Mounting of Stained Materials Procedures in Tissue Preparation for Light Microscopic Cytochemistry-Freezing, Freeze Substitution and Drying Methods, Preparation of Fixatives, Dehydration and Clearing Procedures Procedures for the Cytochemical Localizations of Different Cell Wall Substances Carbohydrates-Insoluble Polysaccharides, Cellulose, Chitin, Callose, Sulphated and Carboxylated polysaccharides Proteins-Non-Specific Cell Wall Proteins, Enzymic Proteins Lignins Sporopollenins Cutins & Suberin Minerals Fluorescent Microscopic Cytochemistry Introduction Fluorescent Cytochemical Methods Cell Wall Carbohydrates Cell Wall Proteins Lignin Ferulic Acid Sporopollenin Cutin and Suberin Transmission Electron Microscopic Cytochemistry Introducti