Methodologies for Metabolomics

Experimental Strategies and Techniques

By: Norbert Lutz (Editor), Jonathan Sweedler (Editor), Ron Wevers (Editor)

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Hardcover | 21 March 2013

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Hardcover
640 Pages

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25.4 x 17.78 x 3.51

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Metabolomics, the global characterisation of the small molecule complement involved in metabolism, has evolved into a powerful suite of approaches for understanding the global physiological and pathological processes occurring in biological organisms. The diversity of metabolites, the wide range of metabolic pathways and their divergent biological contexts require a range of methodological strategies and techniques. Methodologies for Metabolomics provides a comprehensive description of the newest methodological approaches in metabolomic research. The most important technologies used to identify and quantify metabolites, including nuclear magnetic resonance and mass spectrometry, are highlighted. The integration of these techniques with classical biological methods is also addressed. Furthermore, the book presents statistical and chemometric methods for evaluation of the resultant data. The broad spectrum of topics includes a vast variety of organisms, samples and diseases, ranging from in vivo metabolomics in humans and animals to in vitro analysis of tissue samples, cultured cells and biofluids.

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You Can Find This Book In

Non-Fiction Science Biology, Life Sciences Biochemistry Engineering & Technology Biochemical Engineering Biotechnology Chemistry Analytical Chemistry Molecular Biology Medicine Pre-Clinical Medicine & Basic Sciences

Physiology Cellular Physiology

Exploring the human metabolome by NMR spectroscopy and mass spectroscopy
Methodological requirements for lipidomics research
Biological methods for metabolic research
Considerations in sample preparation, collection, and extraction approaches applied in microbial, plant, and mammalian metabolic profiling
MS-based methodologies for single-cell metabolite detection and identification
Direct metabolomics from tissues and cells: laser ablation electrospray ionization for small molecule and lipid characterization
Bioinformatic approaches to process and annotate high-resolution mass spectrometry data
Approaches for natural product detection and structural elucidation using mass spectrometry with high mass accuracy
Metabolomics using ion mobility mass spectrometry
Metabolomics via biomedical mass spectrometry: from sampling to clinical applications
Analytical techniques in metabolomics integrating NMR and chromatography with
NMR of CSF: the neurometabolome
NMR analysis and genetic metabolic disease Udo
NMR methods for metabolomic investigation of amniotic fluid
Chemometric methods in NMR-based body fluid analysis
Lipid profiling in health and disease Christina
NMR-based saliva metabolomics Hanne
Reproducible sample preparation and spectrum acquisition techniques for metabolic profiling of human tissues by
MR spectroscopy in investigating the cancer metabolome in preclinical model systems
Metabolomic magnetic resonance spectroscopy of human tissue: a comparison of in vivo and HR-MAS ex vivo techniques
Phospholipidomics by P NMR spectroscopy of tissue extracts
C NMR for analysis of metabolic pathways
Hyperpolarized NMR spectroscopy - a new method for metabolomic research
Assignment strategies for NMR resonances in metabolomics research
Table of Contents provided by Publisher. All Rights Reserved.

Methodologies for Metabolomics

Experimental Strategies and Techniques

At a Glance

Hardcover

Shipping

How to return your order

Defective items

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