Foreword.
Preface.
Acknowledgments.
Editors and Contributors.
SECTION I: INTRODUCTION.
1 Introduction to Pluripotent Stem Cells: Biology and
Applications (Maria Borowski and Gary S. Stein).
2 Researching and Obtaining Established Stem Cell Lines (Mai
X. Luong, Kelly P. Smith, and Gary S. Stein).
SECTION II: LABORATORY GUIDE FOR HUMAN STEM CELL CULTURE:
PLURIPOTENT STEM CELL CULTURE.
3 Basics of Cell Culture (Alicia Allaire, Mai X. Luong, and
Kelly P. Smith).
4 The Stem Cell Laboratory (Alicia Allaire, Mai X. Luong, and
Kelly P. Smith).
5 Reagent Preparation (Alicia Allaire, Mai X. Luong, and
Kelly P. Smith).
6 Preparation of Mouse Embryonic Fibroblasts for Culture of
Human Embryonic Stem Cells (Meng-Jiao Shi, Maria Borowski, and
Kimberly Stencel).
7 Thawing and Seeding of Frozen Mouse Embryonic Fibroblasts
(Meng-Jiao Shi, Maria Borowski, and Kimberly Stencel).
8 Thawing, Seeding, and Changing the Medium of Human Embryonic
Stem Cells (Meng-Jiao Shi, Kimberly Stencel, and Maria
Borowski).
9 Passaging of Human Embryonic Stem Cells on Inactivated Mouse
Embryonic Fibroblast Plates (Meng-Jiao Shi, Kimberly Stencel,
and Maria Borowski).
10 Harvesting Human Embryonic Stem Cells for Cryopreservation
(Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski).
11 Human Embryonic Stem Cell Culture on BD Matrigel?with
mTeSR®1 Medium (Meng-Jiao Shi, Kimberly Stencel, and Maria
Borowski).
SECTION III: LABORATORY GUIDE FOR HUMAN STEM CELL CULTURE:
CHARACTERIZATION OF PLURIPOTENT STEM CELLS.
12 Defining Pluripotency (Kelly P. Smith and Mai X.
Luong).
13 Characterization of Human Embryonic Stem Cells by
Immunofluorescence Microscopy (Shirwin M. Pockwinse and Prachi
N. Ghule).
14 Preparation of Human Embryonic Stem Cell Samples for Flow
Cytometry (Meng-Jiao Shi, Kimberly Stencel, and Maria
Borowski).
15 Characterization of Human Embryonic Stem Cell Gene Expression
Using Reverse Transcription Polymerase Chain Reaction (Alicia
Allaire, Kelly P. Smith, and Mai X. Luong).
16 Determining Pluripotency of Human Embryonic Stem Cells:
Embryoid Body Formation (Shirwin M. Pockwinse and Prachi N.
Ghule).
17 Characterization of Human Embryonic Stem Cells by
Cytogenetics: Karyotyping and Fluorescence In Situ
Hybridization (Shirwin M. Pockwinse, Prachi N. Ghule and Anne
Higgins).
18 High-Resolution Chromatin Immunoprecipitation Assay
(Beatriz Perez-Cadah?a, Bojan Drobic, and James R.
Davie).
19 Assaying Pluripotency Via Teratoma Formation (M. William
Lensch and Tan A. Ince).
SECTION IV: PERSPECTIVES IN HUMAN STEM CELL
TECHNOLOGIES.
20 Genomic Analysis of Pluripotent Stem Cells (David
Lapointe).
21 Proteomic Analysis of Human Pluripotent Cells (Andy T. Y.
Lau, Yan-Ming Xu, and Jen-Fu Chiu).
22 Bioinformatics Strategies for Understanding Gene Expression
in Human Pluripotent Cells (Gustavo Glusman, Bruz Marzolf, Kai
Wang, Ji-Hoon Cho, Burak Kutlu, and Qiang Tian).
23 Epigenetic Analysis of Pluripotent Cells (Mojgan Rastegar,
Genevieve P. Delcuve, and James R. Davie).
24 Differentiation of Pluripotent Stem Cells: An Overview
(Jeremy Micah Crook).
25 Cellular Reprogramming: Current Technology, Perspectives, and
Generation of Induced Pluripotent Cells (Tanja Dominko).
SECTION V: APPLICATIONS OF HUMAN EMBRYONIC STEM
CELLS.
26 Human Pluripotent Cells: The Biology of Pluripotency
(Li-Fang Chu and Thomas P. Zwaka).
27 Human Pluripotent Cells for Regenerative Medicine: Potential
Applications for Regenerative Medicine (Christopher C. Ford and
Darrell N. Kotton).
28 Therapeutic Applications of Human Embryonic Stem Cells
(Shi-Jiang Lu, Irina Klimanskaya, Edmund Mickunas, and Robert
Lanza).
29 Human Stem Cells for Drug Screening, Toxicity, Specificity,
and Off-Target Effects (Arnaud Lacoste and Mark Burcin).
30 Interfacing Stem Cells with Gene Therapy (Christian
Mueller and Terence R. Flotte).
31 Tissue Engineering for Stem Cell Mediated Regenerative
Medicine (Janet Zoldan, Thomas P. Kraehenbuehl, Abigail K. R.
Lytton-Jean, Robert S. Langer, and Daniel G. Anderson).
Glossary.
Index.
