Homing Endonucleases

1. Homing Endonucleases: From Genetic Anomalies to Programmable Genomic Clippers Marlene Belfort and Richard P. Bonocora 2. Bioinformatic Identification of Homing Endonucleases and Their Target Sites Eyal Privman 3. PCR-Based Bioprospecting for Homing Endonucleases in Fungal Mitochondrial rRNA Genes Mohamed Hafez, Tuhin Kumar Guha, Chen Shen, Jyothi Sethuraman, and Georg Hausner 4. Mapping Homing Endonuclease Cleavage Sites Using In Vitro Generated Protein Richard P. Bonocora and Marlene Belfort 5. Mapping Free-Standing Homing Endonuclease Promoters Using 5'RLM-RACE Ewan A. Gibb 6. PCR Analysis of Chloroplast Double-Strand Break (DSB) Repair Products Induced by I-CreII in Chlamydomonas and Arabidopsis Taegun Kwon, Obed W. Odom, Weihua Qiu, and David L. Herrin 7. A Two-Plasmid Bacterial Selection System for Characterization and Engineering of Homing Endonucleases Ning Sun and Huimin Zhao 8. Rapid Screening of Endonuclease Target Site Preference Using a Modified Bacterial Two-Plasmid Selection Jason M. Wolfs, Benjamin P. Kleinstiver, and David R. Edgell 9. A Yeast-Based Recombination Assay for Homing Endonuclease Activity Jean-Charles Epinat 10. Rapid Determination of Homing Endonuclease DNA Binding Specificity Profile Lei Zhao and Barry L. Stoddard 11. Quantifying the Information Content of Homing Endonuclease Target Sites by Single Base Pair Profiling Joshua I. Friedman, Hui Li, and Raymond J. Monnat, Jr. 12. Homing Endonuclease Target Site Specificity Defined by Sequential Enrichment and Next-Generation Sequencing of Highly Complex Target Site Libraries Hui Li and Raymond J. Monnat, Jr. 13. Homing Endonuclease Target Determination Using SELEX Adapted for Yeast Surface Display Kyle Jacoby and Andrew M. Scharenberg 14. Engineering and Flow-Cytometric Analysis of Chimeric LAGLIDADG Homing Endonucleases from Homologous I-OnuI-Family Enzymes Sarah K. Baxter, Andrew M. Scharenberg, and Abigail R. Lambert 15. Bioinformatics Identification of Coevolving Residues Russell J. Dickson and Gregory B. Gloor 16. Identification and Analysis of Genomic Homing Endonuclease Target Sites Stefan Pellenz and Raymond J. Monnat, Jr. 17. Redesigning the Specificity of Protein-DNA Interactions with Rosetta Summer Thyme and David Baker