Handbook of Phycological Methods

Physiological and Biological Methods

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Paperback | 9 June 2011

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528 Pages

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22.86 x 15.24 x 3.0

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This 1979 volume of the Handbook of Phycological Methods was the first compilation of biochemical and physiological procedures selected specifically for the experimental phycologist. Algae present unique problems to the researcher because of their diverse structure and composition, which differ significantly from those of other commonly used organisms. The book contains practical examples of processes such as photosynthesis, respiration and the transport and accumulation of ions presented by contributors who have applied these these techniques to algae. The methods are described in sufficient detail so that they may be used by technicians, graduate and undergraduate students, and non-specialists. Beginners and experts alike will appreciate the continuing value of this book.

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Non-Fiction Science Biology, Life Sciences Hydrobiology Marine Biology Botany & Plant Sciences Phycology, Algae & Lichens

Contributors
Editors' preface
Introduction
Isolation of Organelles and Membranes
Chloroplasts from Euglena gracilis
Nuclei and chromatin from Euglena gracilis
Mitochondria from Chlorella
Microbodies from Chlorogonium and Spirogyra
Isolation and characterization of diatom membranes
Analysis of Chemical Constituents
Chlorophylls and carotenoids
Algal biliproteins Harold
Nucleic acids
Protein determination by dye binding
Carbohydrate determination by the phenol-sulfaric acid method
Glycerolipids and fatty acids of algae
Carrageenans and agars
ß-1,3-Glucans in diatoms and brown seaweeds
Alginic Acid
Phenolic compounds in brown and red algae
Brown seaweeds: analysis of ash, fiber, iodine and mannitol
Quantitation of the macromolecular components of microalgae
ATP, ADP, and AMP determinations in water samples and algal cultures
Enzymes
Ribulose-bis-phosphate carboxylase in Chlamydomonas
Nitrate reductase in marine phytoplankton
Threonine deaminase in marine microalgae
Phosphoenolpyruvate carboxylse of blue-green algae
Aldolases of multicellular marine algae
The glucosyltranserases of red algae
Anion-activated adenosine triphosphates
Extracellular acid phosphatase of Ocheomonas sanica
Physiological and Biochemical Processes
Determination of photosynthetic rates and 14C photoassimilatory products of brown seaweeds
Polargraphic measurements of respiration following light-dark transitions
Hill reaction and photophosphorylation with chloroplast perparations from Chlamydomonas reinhardi
Respiratory assimilations of 14C-labelled substrates by a microalga
Respiratory processses in mitochondria
Determination of protein synthesis during synchronous growth
Nucleic acid synthesis
Nitrogen fixation
Uptake of organic substrates
Release of organic substances
Nutrients
Nitrate uptake
Phosphate uptake
Sulfate uptake
Uptake of silicic acid
Uptake of micronutrients and toxic metals
Ion Content and Transport
Measurement of ionic content and fluxes in microalgae
Ion transport and ion-stimulated adenosine triphosphatases Cornelius
Inhibitors
Inhibitors used in studies of algal metabolism
Appendix
Indexes
Table of Contents provided by Publisher. All Rights Reserved.

Handbook of Phycological Methods

Physiological and Biological Methods

At a Glance

Paperback

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How to return your order

Defective items

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