Genetic Engineering

Plasmid and phage M13 cloning vectors.- I Introduction.- II Bacterial plasmids.- A Plasmid genes.- B Plasmid replication in E. coli.- C Transfer and mobilization.- III General purpose amplifiable vectors.- A Choice of vector.- B Choice of host cell.- C Selective markers.- D Regeneration of restriction sites.- E The pBR322 series.- IV Specialized vectors.- A Low copy number vectors.- B Vectors designed to detect transcription control signals.- C Direct selection vectors.- D Cosmids.- V Vectors designed to promote gene expression.- A Gene dosage.- B E. coli promoters.- C Fusion proteins.- VI Broad host range vectors.- A E. coli and Gram negatives.- B Bifunctional Bacillus-Escherichia vectors.- VII Single-stranded DNA phages as cloning vectors.- A Filamentous SS DNA phage biology.- B M13 vectors and their uses.- VIII Summary.- IX Acknowledgements.- X References.- Vectors based on bacteriophage lambda.- I Introduction.- II The lambda genome.- A The arrangement of the genes.- B The expression of lambda genes.- III Replication and maturation of lambda DNA.- A DNA structure.- B DNA packaging and size-selection.- C Maturation of recombinant phages.- D In vitro packaging of phage DNA.- IV The recognition of recombinant phages.- A Direct screens.- B Indirect screens.- C Positive selection for recombinant phages.- D Hybridization screening.- E Screens dependent on gene expression.- V The expression of genes cloned into lambda.- A Genes with their own promoter.- B Expression from PL.- C Expression from the late promoter.- D General considerations.- VI Making gene banks with lambda vectors.- VII Conclusions and future developments.- VIII Acknowledgements.- IX References.- Expression of cloned genes in eukaryotic cells using vector systems derived from viral replicons.- I Introduction.- A Why develop eukaryotic cloning systems?.- B What eukaryotic cloning systems are available?.- II Criteria for the design of animal virus vectors.- III Systems for the propagation of recombinant DNA molecules as virions.- A Simian virus 40.- B Human adenoviruses.- C Retroviruses.- IV Episomal vectors based on viral genomes.- A Introduction.- B Papillomavirus vectors.- C Episomal vectors based on Simian virus 40.- V Virus-based vectors carrying selectable genes.- A Introduction.- B The gpt system.- C The aminoglycoside phosphotransferase system.- D The dihydrofolate reductase system.- VI Vectors for the integration of exogenous DNA into chromosomal DNA.- VII Conclusions.- VIII Acknowledgements.- IX References.- A comprehensive list of cloned eukaryotic genes.- Key.- Genome clones.- cDNA clones.