Gene Therapy Protocols

Volume 2: Design and Characterization of Gene Transfer Vectors

By: Joseph LeDoux (Editor)

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Hardcover | 24 April 2008 | Edition Number 3

At a Glance

Hardcover
328 Pages

Edition Type
Revised

Dimensions(cm)
23.5 x 15.88 x 2.54

Hardcover

$249.00

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In Gene Therapy Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. Today's technologies can deliver genetic material safely and effectively to cells to slow or halt the progression of disease, and to help repair or regenerate damaged or lost tissues. In this second volume of Gene Therapy Protocols: Design and Characterization of Gene Transfer Vectors, readers will find a comprehensive resource of current and emerging methods for the processing and characterization of viral and non-viral gene transfer vectors, as well as promising approaches to design vectors for efficient, targeted and regulated gene delivery and expression. This second volume of the new and completely revised third edition of Gene Therapy Protocols will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical researchers in both industry and academia.

Industry Reviews

From the reviews of the third edition:

"This book reflects progress in the field with chapters on early stage vector development and in vitro evaluation, as well as others devoted to issues faced in preclinical and clinical development ... . Readers of this book are likely to be those working in vector development, validation and production as well as those involved in gene-based clinical trials. The specialized nature of this book means that it will primarily be used by experienced researchers ... ." (Christopher Ring, Microbiology Today, June, 2009)

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You Can Find This Book In

Non-Fiction Medicine Clinical & Internal Medicine Engineering & Technology Biochemical Engineering Biotechnology Science Biology, Life Sciences Life Sciences in General Genetics excluding Medical Pre-Clinical Medicine & Basic Sciences Medical Genetics

Science in General Cellular Biology

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Published: 4th November 2014

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Published: 2nd November 2014

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Preface	p. v
Contributors	p. ix
Purification of Retrovirus Particles Using Heparin Affinity Chromatography	p. 1
Scaleable Purification of Adenovirus Vectors	p. 13
Quantifying the Titer and Quality of Adenovirus Stocks	p. 25
Chromatography-Based Purification of Adeno-Associated Virus	p. 37
Spectroscopic Methods for the Physical Characterization and Formulation of Nonviral Gene Delivery Systems	p. 55
Real-Time Multiple Particle Tracking of Gene Nanocarriers in Complex Biological Environments	p. 81
Production of Lentiviruses Displaying "Early-Acting" Cytokines for Selective Gene Transfer into Hematopoietic Stem Cells	p. 99
Fiber-modified Adenoviruses for Targeted Gene Therapy	p. 113
PEGylated Adenovirus for Targeted Gene Therapy	p. 133
Transposon-Based Mutagenesis Generates Diverse Adeno-Associated Viral Libraries with Novel Gene Delivery Properties	p. 161
Photochemical Enhancement of DNA Delivery by EGF Receptor Targeted Polyplexes	p. 171
Reducing the Genotoxic Potential of Retroviral Vectors	p. 183
Evaluation of Promoters for Use in Tissue-Specific Gene Delivery	p. 205
Adenovirus-Mediated Transduction of Auto- and Dual-Regulated Transgene Expression in Mammalian Cells	p. 221
Regulated Expression of Adenoviral Vectors-Based Gene Therapies: Therapeutic Expression of Toxins and Immune-Modulators	p. 239
Liver-Directed Gene Therapy Using the Sleeping Beauty Transposon System	p. 267
Generation and Functional Analysis of Zinc Finger Nucleases	p. 277
Conditional Gene Expression and Knockdown Using Lentivirus Vectors Encoding shRNA	p. 291
Index	p. 311
Table of Contents provided by Ingram. All Rights Reserved.

Gene Therapy Protocols

Volume 2: Design and Characterization of Gene Transfer Vectors

At a Glance

Hardcover

Industry Reviews

Shipping

How to return your order

Defective items

You Can Find This Book In

Other Editions and Formats

Paperback

Paperback

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