Fluorescent Proteins

Volume 85

By: Kevin Sullivan

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Hardcover | 14 December 2007 | Edition Number 2

At a Glance

Hardcover
660 Pages

Edition Type
Revised

Dimensions(cm)
24.13 x 19.05 x 2.54

Hardcover

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This new edition of Fluorescent Proteins presents current applications of autofluorescent proteins in cell and molecular biology authored by researchers from many of the key laboratories in the field. Starting from a current review of the broad palette of fluorescent proteins available, several chapters focus on key autofluorescent protein variants, including spectral variants, photodynamic variants as well as chimeric FP approaches. Molecular applications are addressed in chapters that detail work with single molecules, approaches to generating protein fusions and biosensors as well as analysis of protein-protein interactions in vivo by FRET, fluorescence polarization and fluorescence cross correlation techniques. A number of approaches to in vivo dynamics are presented, including FRAP, photoactivation, and 4-dimensional microscopy. Behavior of spindle components, membrane proteins, mRNA trafficking as well as analysis of cell types in tissues and in development are detailed and provide models for a wide variety of experimental approaches. In addition, several chapters deal directly with the computational issues involved in processing multidimensional image data and using fluorescent imaging to probe cellular behavior with quantitative modeling. This volume brings together the latest perspective and techniques on fluorescent proteins and will be an invaluable reference in a wide range of laboratories.

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You Can Find This Book In

Non-Fiction Science Biology, Life Sciences Molecular Biology Cellular Biology Kids & Children's Books Educational Material Science Educational Material Biology Educational Material Biochemistry Proteins Engineering & Technology

Biochemical Engineering Biotechnology

Fluorescent Proteins
Functional Fusion Proteins by Random Transposon-Based GFP Insertion
Fluorescent Proteins for Photoactivation Experiments
Design and Optimization of Genetically Encoded Fluorescent Biosensors: Gtpase Biosensors
Fast 4D Microscopy
Single molecule imaging of fluorescent proteins
Counting Kinetochore Protein Numbers in Budding Yeast Using Genetically Encoded Fluorescent Proteins
Fluorescent Protein Applications in Plants
Expression and Imaging of Fluorescent Proteins in the C. elegans Gonad and Early Embryo Rebecca
Fluorescent Proteins in Zebrafish Cell and Developmental Biology
Identifying and Quantitating Neural Stem and Progenitor Cells in the Adult Brain
Using Fluorescent Proteins to Study mRNA Trafficking in Living Cells
Visualising mRNA Localisation and Local Protein Translation in Neurons
Quantitative FRAP in Analysis of Molecular Binding Dynamics in Vivo
Quantitative and Qualitative Analysis of Plant Membrane Traffic Using Fluorescent Proteins
Engineering FRET Constructs Using CFP and YFP
Fluorescence Anisotropy Imaging Microscopy (FAIM) for homo-FRET in Living Cells
FRET by Fluorescence Polarization Microscopy
Bimolecular Fluorescence Complementation Visualization of Molecular Interactions in Living Cells
Protein-protein interactions determined by fluorescence correlation spectroscopy
Recent Advances on in Vivo Imaging with Fluorescent Proteins
Computational Processing and Analysis of Dynamic Image Data
Automated Classification of Mitotic Phenotypes of Human Cells Using Fluorescent Proteins
Open Tools for Storage and Management of Quantitative Image Data
Table of Contents provided by Publisher. All Rights Reserved.

Fluorescent Proteins

Volume 85

At a Glance

Hardcover

Shipping

How to return your order

Defective items

You Can Find This Book In

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