Enzyme Engineering

Keynote Paper.- Achievements and Predicted Developments in Enzyme Engineering.- Session I. Enzyme Production.- Comparative Quantitative Physiology of High Cellulase Producing Strains of Trichoderma Reesei.- Bacterial Exo-Enzyme and Exo-Toxin Export.- Enzyme Purification by Liquid-Liquid Extraction.- Continuous Isolation of Yeast-Lytic Enzymes from Citophaga.- Characterization of Membranes for Enzyme Retention.- Cloning of the Penicillin G-Acylase Gene of Escherichia coli ATCC 11105 on Multicopy Plasmids.- Immobilized Tannin as Protein Adsorbent-Preparation, Characteristics, and Applications.- Aminoalkylpolysaccharide Sorbents in Purification of Enzymes.- Immunochemical Identification of a New Thermostable ?-Galactosidase from a Bacillus Species.- Mass Spectrometer Monitoring of a Yeast Fermentation.- Affinity Chromatographic Investigations on Enzyme Coenzyme Complexes of CoA-Dependent Enzymes.- Session II. Enzymes and Energy Transduction.- Principles of Bioelectrocatalysis.- Cofactor Modified Electrodes for Energy Transfer.- Immobilized Thylakoids and Chromatophores: Hydrogen Production and ATP Regeneration.- Some Aspects of Direct Bioelectrocatalytic Regeneration of Cofactors.- A New Enzyme Transducer Combination: The Enzyme Transistor.- A Simple, General Method for Preparation of Hydrosoluble Polymeric Adenine Nucleotide Coenzymes.- Cofactor Reaction-Diffusion Kinetics for an Alcohol Dehydrogenase Membrane.- Approaches to Stabilization of Hydrogenase and Nitrogenase Against Oxygen Inactivation.- Biochemical Energy Conversion System.- Session III: Biomass Conversion.- Utilization of ?-Glucosidase from Aspergillus Species in the Hydrolysis of Cellulose.- The Comparative Role of Exoglucosidase and Cellobiase in Glucose Formation from Cellulose.- Novel Enzymes from Methylotrophic Microorganisms.- Ethanol - Butanediol Fermentation of Xylose.- Fluorimetric Monitoring of Methanogenesis in Anaerobic Digesters.- Session IV: Biomedical and Analytical Applications of Enzymes and Antibodies.- Enzyme Channelling Immunoassay. A New Homogeneous Enzyme Immunoassay Technique.- Human Renal Renin: Purification, Production of Specific Antibodies. Clinical Applications.- Competitive Binding Studies of Carbohydrate-Lectin Interactions Using a Lectin Electrode.- Potentiometrie Enzymatic Measurement of Glucose: Possible In Vivo Use.- Immobilized Enzyme Pipette - "Impette".- Electrochemical Determination of Lipids in Serum.- Immobilized Enzymes for Medical Application.- Photoenzography: Using Enzymes for Photographic Aims.- The Design of Peroxide Enzyme Membrane Polarographic Sensors for Clinical and Industrial Analysis.- New Approaches Using Immobilized Enzymes for the Removal of Urea and Ammonia.- Enzyme-Collagen Reactor Designed from an Hemodialysis Module.- Immobilization and Characterization of Enzymes on Hollow Fibers for a Possible Use in the Biomedical Field.- Magnetic Microspheres for Targeting of Drugs.- Modification of Enzymes with Water-Soluble Polymers.- Detoxication of Ammonia by Immobilized Urea Cycle Enzymes.- Use of the Enzyme Thermistor for Continuous Monitoring and Control in Biotechnology.- Distribution of Damkohler Number of Spherical Matrix Particles by Image Analysis.- On-line Determination of Reaction Rate Versus Substrate Concentration for an Enzymatically Catalysed Reaction by Means of a Microcomputer System.- Microbial Sensors for Ethyl Alcohol, Acetic Acid and Ammonia.- Session V: Large-Scale Transformations Using Bound Enzymes.- Improved Whey Treatment by Immobilized Lactase.- Lactose Hydrolysis by Immobilized Lactase: Semi-industrial Experience.- Production of Useful Nucleotides with Immobilized Microbial Cells.- Heterogeneous Biocatalysis in the Degradation of ?-Glucan Polymers.- Absorption Into a Liquid Film in Laminar Flow Coupled with an Enzyme Catalyzed Reaction at the Solid Surface.- Catalyst Deactivation and Optimal Operation Policy: Immobilized ?-galactosidase.- Stability Studies on the Immobilized Glucose Oxidase/Catalase Enzyme System.- Hydrodynamics and Kinetics of Hollow Fiber Enzyme Reactors.- Isomerose Syrups Containing Greater than 90% Fructose.- Session VI: Immobilized Cells and Organelles.- Immobilization of Whole Microbial Cells for the Production of 6-Amino Penicillanic Acid.- Application of Immobilized Biocatalysts to Bioconversion in Hydrophobic Conditions.- Alginate Bead Entrapped Yeast Cells for Continuous Inversion of Sucrose and Molasses.- Physical Characterization of Biocatalyst Particles Obtained form Polymer Entrapment of Whole Cells.- The Use of Free and Immobilized Cells in the Presence of Organic Solvents: The Oxidation of Cholesterol by Nocardia Rhodochrous.- Properties of Enzymes Solubilized in Hydrocarbons via Reversed Micelles.- The Potential Use of Immobilized Plant Cells for the Production and Transformation of Natural Products.- Immobilized Whole Cells of the Yeast Trigonopsis variabilis Containing D-Amino Acid Oxidase for the Production of ct-Keto Acids.- Enzymatic Synthesis of Pantothenic Acid by Escherichia coli Cells.- Production of Useful Chemicals Using Cells Immobilized with Polyacrylamide and Carrageenan.- Affinity Chromatographic Purification of Proteins Using Immobilized Cells.- Synthesis of Coenzymes by Immobilized Cell System.- Session VII: Bound Enzymes and the Synthesis of Fine Chemicals and Pharmaceuticals.- Continuous Deacetylation of Cephalosporins.- Thermostability of Soluble and Immobilized Subtilisins After Their Modification by Dextrans and Dextrins.- Isolation and Immobilization of Porcine Ligandin with Glutathione Transferase Activity.- Studies on the Stability of Soluble and Immobilized Alcohol Dehydrogenase from Yeast Mitochondria.- Distribution of Staphylococcal Nuclease Insolublizied on Sepharose.- Immobilized Penicillin Acylase for Production of 6-APA From Penicillin-V.- Preparation and Characterization of Metal Oxide-coated Supports for Oxidase Enzymes.- Enzyme Immobilization on Pyridine Containing Polymers.- Cross-linked Pectate.- Immobilization of Biocatalysts Using Ultrafiltration Techniques.- ?-Galactosidase Immobilized on Benzoquinone-Activated Bead Cellulose and on Oxirane-Acrylic Beads.- Application of Immobilized Carboxypeptidase Y for Deblocking in Peptide Synthesis.- Adsorption and Distribution of Enzymes in Carriers.- Application of Photosensitive Immobilized ?-Chymotrypsin to Synthetic Reaction.- List of Participants.