Drug Absorption Studies

Part 1: Perfused Organ Level/In Situ Techniques: Models for skin absorption and skin toxicity testing -- Models of the small intestine -- Drug absorption from the colon in situ -- In vivo and ex vivo models for assessing drug delivery across the buccal mucosa -- In situ and ex vivo nasal models for preclinical drug development studies -- The isolated perfused lung for drug absorption studies -- Part 2: Cellular Level - In Vitro Models of Epithelial and Endothelial Barriers: In vitro models for investigations of buccal drug permeation and metabolism. -- In vitro screening models to assess intestinal drug absorption and metabolism -- In vitro cellular models for nasal drug absorption studies -- In vitro models of the bronchial epithelium -- In vitro models of the alveolar epithelial barrier -- Cell culture models of the corneal epithelium and reconstructed cornea equivalents for in vitro drug absorption studies -- The conjunctival barrier in ocular drug delivery -- Inner blood-retinal barrier: transport biology and methodology -- Regulation of paracellular permeability in low-resistance human vaginal-cervical epithelia -- In vitro models and multidrug resistance mechanisms of the placental barrier -- In vitro models to study blood-brain barrier function -- High-throughput screening using Caco-2 cell and PAMPA systems -- Instrumented approaches for the assessment of drug transport across intestinal and pulmonary epithelial barriers -- Part 3: Bioinformatics - In Silico Tools to Predict Drug Absorption: Modelling transdermal absorption -- Physiologically based in silico models for the prediction of oral drug absorption -- In silico modeling for blood-brain barrier permeability predictions -- Part 4: Molecular/Sub-Cellular Level - Mechanistic Tools: Impact of drug transport proteins -- Cloning and functional heterologous expression of transporters -- Pharmacology of caveolae -- Immortalization strategies for epithelial/endothelial cells in primary culture -- Binding-uptake studies and cellular targeting -- Part 5: Regulatory considerations: Drug permeability studies in regulatory biowaiver applications.