Cytochemical Staining Methods for Electron Microscopy

Chapter 1. Introduction (P.R. Lewis and D.P. Knight). 1.1. The Objectives of Section Staining. 1.2. Contrast in Transmission Electron Microscopy. 1.3. Fixation of tissues. 1.4. Preparation of Tissues. 1.5. Special Embedding Techniques. 1.6. Safety in the Laboratory. Chapter 2. Cytological Staining Methods (D.P. Knight and P.R. Lewis). 2.1. Resin Sections for Light Microscopy. 2.2. Procedures for Handling Ultrathin Sections. 2.3. Lead Stains. 2.4. Uranyl Acetate. 2.5. A Procedure for Double Staining. 2.6. Osmium Tetroxide. 2.7. Other Cytological Stains. 2.8. Tracers in Electron Microscopy. Chapter 3. General Cytochemical Methods (D.P. Knight and P.R. Lewis). 3.1. The limitations of present techniques. 3.2. Cytochemical Methods with Broad Specificities. 3.3. Methods for Nucleic Acids. 3.4. General Methods for Proteins. 3.5. Specific Methods for Carbohydrates. 3.6. Methods for Lipids. 3.7. Precipitation Methods for Inorganic Ions. 3.8. Extractive Methods. Chapter 4. Metal Precipitation Methods for Hydrolytic Enzymes (P.R. Lewis). 4.1. The Principles of Enzyme Cytochemistry. 4.2. Some Practical Problems of Enzymes Cytochemistry. 4.3. Methods for Acid Hydrolases and Related Techniques. 4.4. Enzymes Which Hydrolyze ATP. 4.5. Other Phosphatases. 4.6. General Techniques for Esterases. 4.7. Techniques Specific for the Cholinesterases. Chapter 5. Other Cytochemical Methods for Enzymes (P.R. Lewis). 5.1. Histochemistry of the Oxidases. 5.2. A Biochemical Background to Dehydrogenase Histochemistry. 5.3. Ferricyanide as a Histochemical Reagent. 5.4. The Use of Tetrazolium Salts. 5.5. Azo-dye Coupling Techniques. 5.6 Methods for Other Enzymes. 5.7. Recent Advances and Future Development in Cytochemistry. Appendix 1. Stock solutions. Appendix 2. List of suppliers.