Binding Constants

The Measurement of Molecular Complex Stability

By: Kenneth A. Connors

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Hardcover | 20 April 1987 | Edition Number 1

At a Glance

Hardcover
432 Pages

Dimensions(cm)
23.65 x 15.55 x 2.81

Hardcover

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This unique book presents a systematic review of the methods for the determination of binding constants of complex formation in solution. Material has been gathered which has been scattered throughout the literature of several separate fields. Offered here are methods from the areas of acid-based chemistry, metal- ion coordination compounds, hydrogen-bonding, charge-transfer complexation, hydrophobic interaction and protein-binding. The relevant thermodynamics, modelling, statistics and regression analysis, and interpretation of data are discussed, along with fresh discussions of random association (contact complexes), selection of standard states and comparison of results. All of the experimental methods useful for measuring these equilibrium constants are treated, including those based on spectrophotometry, nuclear magnetic resonance, reaction kinetics, potentiometry, solubility, liquid-liquid partitioning, dialysis, chromatography, flourimetry and many others.

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