Antibody Engineering Volume 1

By: Roland E. Kontermann (Editor), Stefan Dubel (Editor)

Write A Review

Hardcover | 26 March 2010 | Edition Number 2

At a Glance

Hardcover
804 Pages

Edition Type
Revised

Dimensions(cm)
25.4 x 17.8 x 3.81

Hardcover

$539.00

or 4 interest-free payments of $134.75 with

or

Ships in 5 to 7 business days

Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development.

Shipping

	Standard Shipping	Express Shipping
Metro postcodes:	$9.99	$14.95
Regional postcodes:	$9.99	$14.95
Rural postcodes:	$9.99	$14.95

Orders over $79.00 qualify for free shipping.

How to return your order

At Booktopia, we offer hassle-free returns in accordance with our returns policy. If you wish to return an item, please get in touch with Booktopia Customer Care.

Additional postage charges may be applicable.

Defective items

If there is a problem with any of the items received for your order then the Booktopia Customer Care team is ready to assist you.

For more info please visit our Help Centre.

More in Medical Research

Dopamine Nation

Finding Balance in the Age of Indulgence

Paperback

RRP $22.99

$20.75

10%
OFF

Magic Pill

The Extraordinary Benefits and Disturbing Risks of the New Weight Loss Drugs

Paperback

RRP $34.99

$13.00

63%
OFF

The Immortal Life of Henrietta Lacks : Picador Collection - Rebecca Skloot

$62.75

12%
OFF

LYMPH & LONGEVITY

The Untapped Secret to Health

Paperback

RRP $32.99

$25.75

22%
OFF

The Pfizer Papers : Pfizer's Crimes Against Humanity - The WarRoom/DailyClout Pfizer Documents Analysts

$22.99

15%
OFF

Magic Pill

How the New Weight Loss Drugs Will Change Your Life - and the World

Paperback

RRP $26.99

$21.75

19%
OFF

Good Energy

The Surprising Connection Between Glucose, Metabolism and Limitless Health

Paperback

RRP $34.99

$28.75

18%
OFF

Systematic Approaches to a Successful Literature Review - Andrew Booth

$77.75

13%
OFF

Anticancer: A New Way of Life

A New Way of Life

Paperback

RRP $39.99

$31.75

21%
OFF

Essentials of Medical Statistics

2nd Edition

Paperback

RRP $91.95

$59.99

35%
OFF

Munro's Statistical Methods for Health Care Research

Revised 6th Edition

Paperback

RRP $276.99

$186.99

32%
OFF

Essentials of Biostatistics in Public Health

4th Edition

Paperback

RRP $174.75

$108.99

38%
OFF

ABC of Clinical Reasoning

ABC Series

Paperback

RRP $55.95

$42.75

24%
OFF

Introduction to Research in the Health Sciences

7th Edition

Paperback

RRP $76.95

$61.75

20%
OFF

Washington Manual of Medical Therapeutics - Washington University

$42.75

22%
OFF

The Great Prostate Hoax : How Big Medicine Hijacked the PSA Test and Caused a Public Health Disaster - Richard J. Ablin

$11.00

63%
OFF

Designing Clinical Research

5th edition

Paperback

RRP $188.99

$160.64

15%
OFF

Cloning of the Antigen-binding Site from Hybridoma
Cloning of Variable Domains from Mouse Hybridoma by PCR	p. 3
Coning Hybridoma cDNA by RACE	p. 15
Construction of scFv Fragments from Hybridoma or Spleen Cells by PCR Assembly	p. 21
Generation of Antibody Repertoires
Mouse Immune Libraries for the Generation of ScFv Fragments Directed Against Human Cell Surface Antigens	p. 47
Human Antibody Gene Libraries	p. 65
Synthetic Antibody Libraries	p. 85
Immune Libraries from Nonhuman Primates (NHP)	p. 99
Generation of Rabbit Immune Libraries	p. 115
Selection of Antibody Fragments from Combinatorial Libraries
Immunotube Selections	p. 127
Phage Display and Selection in Microtitre Plates	p. 139
Phage Display and Selections on Biotinylated Antigens	p. 151
Phage Display and Subtractive Selection on Cells	p. 165
Selection of Phage Antibody Libraries for Binding and Internalization into Mammalian Cells	p. 183
Improving Phage Display Throughput by Using Hyperphage, Miniaturized Titration and pVIII (g8p) ELISA	p. 197
Yeast Display and Selections	p. 207
The Generation of Transgenic Mice Expressing Human Antibody Repertoires	p. 235
Selection of Antibody Fragments by Means of the Filter-Sandwich Colony Screening Assay	p. 255
Semi-automated Magnetic Bead-Based Antibody Selection from Phage Display Libraries	p. 267
Engineering and Production of Immunoglobulins
Aspects of Isotype Selection	p. 291
Generation of Heavy and Light Chains (Chimeric Antibodies)	p. 307
Humanising Antibodies by CDR Grafting	p. 319
Humanization by Resurfacing	p. 341
Human Antibodies by Guided Selection	p. 355
In Silico De-Immunization	p. 369
Affinity Maturation by Chain Shuffling and Site Directed Mutagenesis	p. 377
Affinity Maturation by Random Mutagenesis and Phage Display	p. 397
Engineering of the Fc Region for Improved PK (FcRn Interaction)	p. 411
Antibody-Dependent Enzyme Prodrug Therapy (ADEPT)	p. 431
Production of Recombinant Human IgG Antibodies in the Baculovirus Expression System	p. 453
Expression of IgA Molecules in Mammalian Cells	p. 471
Antibody Characterization
Expression of Complete Antibodies in Transgenic Plants	p. 489
Expression of Full Length Monoclonal Antibodies (mAb) in Algal Chloroplast	p. 503
Expression of IgG Antibodies in Mammalian Cells	p. 517
Protein A/G Chromatography	p. 531
Epitope Analysis Using Synthetic Peptide Repertoires Prepared by SPOT Synthesis Technology	p. 537
Epitope Mapping by Printed Peptide Libraries	p. 573
Antibody Epitope Mapping Using Yeast Display	p. 591
Size Exclusion Chromatography	p. 607
Structural Characterization of Antibodies by Mass Spectrometry	p. 613
Antibody Glycans Characterization	p. 635
Affinity Measurements by Competition ELISA	p. 657
Anti-Histidine Antibodies as Tools for Reversible Capturing of His-Tagged Fusion Proteins for Subsequent Binding Analysis	p. 667
Affinity Measurements Using Quartz Crystal Microbalance (QCM)	p. 683
Affinity Measurements with Radiolabeled Antibodies	p. 695
Neutralization Tests	p. 705
Functional Characterization of Antibodies Neutralizing Soluble Factors In Vitro and In Vivo	p. 723
Competitive ELISA	p. 739
Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA	p. 743
Determination of Fc-Mediated Antibody-Effector Functions by Chromium Release Assay	p. 749
Binding Studies with Flow Cytometry	p. 765
Index	p. 781
Table of Contents provided by Ingram. All Rights Reserved.

Antibody Engineering Volume 1

At a Glance

Hardcover

Shipping

How to return your order

Defective items

You Can Find This Book In

Other Editions and Formats

Paperback

More in Medical Research

Dopamine Nation

Finding Balance in the Age of Indulgence

Magic Pill

The Extraordinary Benefits and Disturbing Risks of the New Weight Loss Drugs

The Immortal Life of Henrietta Lacks

Picador Collection

Dermatology

Current Developments and Future Prospects

Recent Developments in Pulmonary Inflammation

Introduction to Medical Statistics

4th edition

LYMPH & LONGEVITY

The Untapped Secret to Health

The Pfizer Papers

Pfizer's Crimes Against Humanity

Inflamed

Deep Medicine and the Anatomy of Injustice

Magic Pill

How the New Weight Loss Drugs Will Change Your Life - and the World

Good Energy

The Surprising Connection Between Glucose, Metabolism and Limitless Health

Systematic Approaches to a Successful Literature Review

Causal Inference

What If

Anticancer: A New Way of Life

A New Way of Life

Essentials of Medical Statistics

2nd Edition

Munro's Statistical Methods for Health Care Research

Revised 6th Edition

Essentials of Biostatistics in Public Health

4th Edition

ABC of Clinical Reasoning

ABC Series

Introduction to Research in the Health Sciences

7th Edition

Washington Manual of Medical Therapeutics

Quantum Biology

A glimpse into the future of medicine

The Great Prostate Hoax

How Big Medicine Hijacked the PSA Test and Caused a Public Health Disaster

On the Scent

Unlocking the Mysteries of Smell - and How Its Loss Can Change Your World

Designing Clinical Research

5th edition

This product is categorised by