Analytical and Preparative Separation Methods of Biomacromolecules

Analysis of HbA and some Hb variants by HPLC; analysis of posttranslational modifications in recombinant proteins by HPLC and mass spectrometry; analytical and preparative separations of peptides by capillary and free-flow zone electrophoresis; bioaffinity chromatography; characterization and partial purification of steroidongenic factors from thymic epithelial cell-conditioned medium; determination of affinity constants of lectins for sugars by affinity capillary electrophoresis; displacement chromatography of biomolecules; high performance membrane chromatography of proteins; HPLC purification of recombiant proteins; HPLC purification of recombiant proteins; isolation, purification, and characterization of human seminal plasma proteins and their immunological behaviour in vitro; isolation, purification, and structural study of allergenic proteins; multiangle light scattering combined with HPLC with examples for biopolymers; purification and characterization of connective tissue growth factor using heparin affinity chromatography; slalom chromatography - a new hydrodynamic-based chromatographic mode applicable to size-dependent separation and physiochemical analysis of large DNA molecules; simultaneous chromatographic separation of ceruloplasmin and serum amine oxidase; fast, single-step affinity chromatography purification of haemoglobin.