| Preface | p. v |
| Contributors | p. xi |
| Resources for Genetic and Genomic Studies of Xenopus | p. 1 |
| The Physiology of the Xenopus laevis Ovary | p. 17 |
| Oocyte Isolation and Enucleation | p. 31 |
| Xenopus tropicalis Oocytes: More Than Just a Beautiful Genome | p. 43 |
| Oocyte Expression With Injection of Purified T7 RNA Polymerase | p. 55 |
| Visualization of the Cytoskeleton in Xenopus Oocytes and Eggs by Confocal Immunofluorescence Microscopy | p. 69 |
| Multiphoton Laser Scanning Microscopy as a Tool for Xenopus Oocyte Research | p. 87 |
| Imaging Ca2+ Signals in Xenopus Oocytes | p. 103 |
| Chromosomal DNA Replication in a Soluble Cell-Free System Derived From Xenopus Eggs | p. 121 |
| Chromatin Assembly of DNA Templates Microinjected Into Xenopus Oocytes | p. 139 |
| Pre-mRNA Splicing in the Nuclei of Xenopus Oocytes | p. 149 |
| Chromatin Immunoprecipitation for Studying Transcriptional Regulation in Xenopus Oocytes and Tadpoles | p. 165 |
| Cytoplasmic mRNA Polyadenylation and Translation Assays | p. 183 |
| Xenopus Egg Extracts: A Model System to Study Proprotein Convertases | p. 199 |
| Analysis of Molecular Chaperones Using a Xenopus Oocyte Protein Refolding Assay | p. 213 |
| Ubiquitin-Mediated Protein Degradation in Xenopus Egg Extracts | p. 223 |
| Introduction to Nucleocytoplasmic Transport: Molecules and Mechanisms | p. 235 |
| Use of Xenopus laevis Oocyte Nuclei and Nuclear Envelopes in Nucleocytoplasmic Transport Studies | p. 259 |
| Nuclear Pore Complex Structure and Plasticity Revealed by Electron and Atomic Force Microscopy | p. 273 |
| In Vitro Study of Nuclear Assembly and Nuclear Import Using Xenopus Egg Extracts | p. 289 |
| Use of Intact Xenopus Oocytes in Nucleocytoplasmic Transport Studies | p. 301 |
| Studying the Mechanosensitivity of Voltage-Gated Channels Using Oocyte Patches | p. 315 |
| Oocytes as an Expression System for Studying Receptor/Channel Targets of Drugs and Pesticides | p. 331 |
| Microtransplantation of Neurotransmitter Receptors From Cells to Xenopus Oocyte Membranes: New Procedure for Ion Channel Studies | p. 347 |
| Reconstitution of Golgi Disassembly by Mitotic Xenopus Egg Extracts in Semi-Intact MDCK Cells | p. 357 |
| Exploring RNA Virus Replication in Xenopus Oocytes | p. 367 |
| Study of Apoptosis in Vitro Using the Xenopus Egg Extract Reconstitution System | p. 379 |
| Studying Fertilization in Cell-Free Extracts: Focusing on Membrane/Lipid Raft Functions and Proteomics | p. 395 |
| Localized Sampling, Electrophoresis, and Biosensor Analysis of Xenopus laevis Cytoplasm for Subcellular Biochemical Assays | p. 413 |
| Monitoring Protein Kinase A Activities Using Expressed Substrate in Live Cells | p. 425 |
| Using Xenopus Oocyte Extracts to Study Signal Transduction | p. 435 |
| Oocyte Extracts for the Study of Meiotic M-M Transition | p. 445 |
| Methods for Studying Spindle Assembly and Chromosome Condensation in Xenopus Egg Extracts | p. 459 |
| Index | p. 475 |
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