Techniques in Molecular Systematics and Evolution

By: Rob DeSalle (Editor), Gonzalo Giribet (Editor), Ward Wheeler (Editor)

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Paperback | 1 April 2002

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424 Pages

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The amount of information that can be obtained by using molecular techniques in evolution, systematics and ecology has increased exponentially over the last ten years. The need for more rapid and efficient methods of data acquisition and analysis is growing accordingly. This manual presents some of the most important techniques for data acquisition developed over the last years. The choice and justification of data analysis techniques is also an important and critical aspect of modern phylogenetic and evolutionary analysis and so a considerable part of this volume addresses this important subject. The book is mainly written for students and researchers from evolutionary biology in search for methods to acquire data, but also from molecular biology who might be looking for information on how data are analyzed in an evolutionary context. To aid the user, information on web-located sites is included wherever possible. Approaches that will push the amount of information which systematics will gather in the next few years by using high throughput methods and access to genomics information are also discussed in the manual. In summary, this book is a comprehensive account of practical vs theoretical and modern vs future achievements of molecular systematics in evolutionary biology.

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Non-Fiction Science Biology, Life Sciences Molecular Biology Biochemistry Life Sciences in General Ecological Science Evolution Medicine Medicine in General Public Health & Preventive Medicine Other Branches of Medicine

Pathology Medical Microbiology & Virology Physics Atomic & Molecular Physics

List of Contributors	p. VII
General Introduction	p. IX
Analytical Methods
Introduction to Part I	p. 3
Phylogenetic Analysis: The Basic Method	p. 4
Parsimony Analysis	p. 31
Optimization Alignment: Down, Up, Error, and Improvements	p. 55
Techniques for Analyzing Large Data Sets	p. 70
Measures of Support	p. 80
Partitioning of Multiple Data Sets in Phylogenetic Analysis	p. 102
Complex Model Organism Genome Databases	p. 120
Comparative Phylogenomics: A Strategy for High-throughput Large-scale Sub-genomic Sequencing Projects for Phylogenetic Analysis	p. 132
Comparative Methods	p. 146
Analyzing Data at the Population Level	p. 162
Laboratory Methods
Introduction to Part II	p. 175
Obtaining, Storing and Archiving Specimens and Tissue Samples for Use in Molecular Studies	p. 176
DNA Isolation Procedures	p. 249
Late Pleistocene DNA Extraction and Analysis	p. 288
PCR Methods and Approaches	p. 302
High-Throughput DNA Sequencing for Systematics	p. 328
A Practical Guide for Microsatellite Analysis	p. 351
Determining the Spatial and Temporal Patterns of Developmental Gene Expression in Vertebrates and Invertebrates Using in situ Hybridization Techniques	p. 365
Guide to Solutions	p. 395
Guide to protocols	p. 397
Troubleshooting Guide	p. 399
Index	p. 401
Table of Contents provided by Syndetics. All Rights Reserved.

Techniques in Molecular Systematics and Evolution

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