Part I Introduction 1. Synthetic mRNA: Production, Introduction into Cells, and Physiological Consequences Robert E. Rhoads Part II Synthesis of mRNA 2. Synthetic Capped mRNAs for Cap-Specific Photo-Crosslinking Experiments Joanna Kowalska, Franck Martin, and Jacek Jemielity "/i> 3. Enzymatic Modification of 5'-Capped RNA and Subsequent Labeling by Click Chemistry Josephin M. Holstein, Daniela Stummer, and Andrea Rentmeister 4. Preparation of Functional, Fluorescently Labeled mRNA Capped with Anthraniloyl-m7GpppG Artem V. Domashevskiy, David J. Rodriguez, Dilantha Gunawardana, and Dixie J. Goss 5. Intronless b-Globin Reporter: A Tool for Studying Nuclear RNA Stability Elements Jessica A. Brown and Joan A. Steitz 6. Synthetic mRNA with Superior Properties that Mimics the Intracellular Fates of Natural Histone mRNA Wei Su, Michael K. Slevin, William F. Marzluff, and Robert E. Rhoads 7. Engineering WT1-Encoding mRNA to Increase Translational Efficiency in Dendritic Cells Daphne Benteyn, Carlo Heirman, Kris Thielemans, and Aude Bonehill Part III Introduction of Synthetic mRNA into Cells 8. Electroporation of Alphavirus RNA Translational Reporters into Fibroblastic and Myeloid Cells as a Tool to Study the Innate Immune System Christina L. Gardner, Derek W. Trobaugh, Kate D. Ryman, and William B. Klimstra 9. GMP-Grade mRNA Electroporation of Dendritic Cells for Clinical Use Judith Derdelinckx, Zwi N. Berneman, and Nathalie Cools 10. Large Scale mRNA Transfection of Dendritic Cells by Electroporation in Continuous Flow Systems David Selmeczi, Thomas Steen Hansen, Ozcan Met, Inge Marie Svane, and Niels B. Larsen 11. FLT3 Ligand as a Molecular Adjuvant for Naked RNA Vaccines Sebastian Kreiter, Mustafa Diken, Abderraouf Selmi, Jutta Petschenka, Ozlem Tureci, and Ugur Sahin 12. Transfecting Human Monocytes with RNA Jens Dannull and Smita K. Nair 13.< In Vitro Synthesis, Delivery and Bioavailability of Exogenous mRNA in Gene Transfer Mediated by PiggyBac Transposition Solenne Bire, Nicole Ishac, and Florence Rouleux-Bonnin 14. Transfection of Human Keratinocytes with Nucleoside-Modified mRNA Encoding CPD-Photolyase to Repair DNA Damage Gabor Boros, Katalin Kariko, Hiromi Muramatsu, Edit Miko, Eszter Emri, Csaba Hegedus, Gabriella Emri, and Eva Remenyik Part IV Alteration of Cellular Function with Synthetic mRNA 15. Delivery of Synthetic mRNA Encoding FOXP3 Antigen into Dendritic Cells for Inflammatory Breast Cancer Immunotherapy Gayathri R. Devi and Sritama Nath 16. Immune Monitoring using mRNA-Transfected Dendritic Cells Troels Holz Borch, Inge Marie Svane, and Ozcan Met 17. Transfection of Tumor-Infiltrating T Cells with mRNA Encoding CXCR2 Manja Idorn, Per thor Straten, Inge Marie Svane, and Ozcan Met 18. mRNA Electroporation of Dendritic Cells with WT1, Survivin and TriMix (A Mixture of caTLR4, CD40L, and CD70) An Coosemans, Sandra Tuyaerts, Kim Morias, Jurgen Corthals, Carlo Heirman, Kris Thielemans, Stefaan W. Van Gool, Ignace Vergote, and Frederic Amant 19. Redirecting T Cell Specificity Using T Cell Receptor Messenger RNA Electroporation Sarene Koh, Noriko Shimasaki, and Antonio Bertoletti 20. Measuring Hematocrit in Mice Injected with In Vitro-Transcribed Erythropoietin mRNA Azita Josefine Mahiny and Katalin Kariko 21. Genetic Modification of Human Pancreatic Progenitor Cells through Modified mRNA Song Lu, Christie C. Chow, Junwei Zhou, Po Sing Leung, Stephen K. Tsui, and Kathy O. Lui
