Solving Problems with Microscopy

List of Contributors x

Foreword xii

Preface xvi

Abbreviations xvii

Introduction 1

1 Discovery with the Light Microscope 8

1.1 Hooke, Leeuwenhoek and the Single Lens 10

1.2 Single-lens Microscopes come of Age 14

1.3 Light Microscopes in the Modern Age 17

2 When Problem Solving, Exercise the Scientific Method at Every Step 22

2.1 The Buttonier Case 23

2.2 The Leaky Polio Virus Dispettes 28

2.3 The Green River Killer 32

2.4 The Unfortunate Failure of the Dragline Excavator 40

2.5 The Bodega Burglary 42

3 Images Are Real Data, Too 46

3.1 Mesothelioma Linked to Asbestos 49

3.2 Talc Case 54

3.3 Ford Pinto Case 57

3.4 Uncovering a Moose Hair Cover-up 61

3.5 Carbon Black and Tire Rubber Problems 63

3.6 Optical Microscopy Takes Center Stage: Melamine in Pet Food 67

3.7 Characterization of Foreign Particulate in Pharmaceuticals 77

4 The Microscope as a Compass 87

4.1 Hair Extension Case 88

4.2 Blue Yarn Case 91

4.3 eBay Evidence 93

4.4 An Attractive Contamination 96

4.5 Identifying Metallic Particulates in Pharmaceutical Sample Holders 97

4.6 15th-Century Block Books at The Morgan Library & Museum: The Role of Microscopy in Unraveling Complex Ink Formulations 104

4.7 The Critical Value of Microscopy within Pharmaceutical Development 110

5 Rely on the Fundamentals 120

5.1 A Mouse, a Soft Drink Can ⦠and a Felony 121

5.2 Goodrich Corrosion Problem 129

5.3 The Perils of Forgetting the Fundamentals in Criminal Cases 131

5.4 Super Bowl White Powder Attacks 2014 134

5.5 College Drug Party 137

6 Fortune Favors the Prepared Mind 139

6.1 The NASA Problem 140

6.2 A Train Engine Contamination 142

6.3 Mianus River Bridge Collapse: Why Do You Need a Microscope to Determine Why a Bridge Fell Down? 145

6.4 Microcrystals Tests for Drugs Using the Chemical Microscope (PLM) 152

7 Know Your Limitations 157

7.1 Why Does Guercino's Samson Captured by the Philistines Have a Grainy Surface Texture in Some Paint Passages? 158

7.2 The Secrets of Hair 163

7.3 A Connecticut Murder Case 171

8 The Resonance Theory of Experiments 175

8.1 The Red Hooded Sweatshirt 176

8.2 Florida Arson Case 179

8.3 The Multimillion-Dollar Waterproof Failure 182

9 Value of Multiple Associations 202

9.1 Atlanta Child Murders Investigation 203

9.2 Hog Trail Murders 210

9.3 Hoeplinger Murder 213

9.4 Jackson Pollock Authentication 221

10 Defining Meaningful Differences 238

10.1 The Yellow Rope 240

10.2 Lightning Strike 243

10.3 Raman Microprobe Characterization of ZrO2 Inclusions in Glass Lightguides 247

10.4 Whose Soot Is It Anyway? 253

11 The Importance of Context 267

11.1 GE Capital White-Powder Case 268

11.2 XB-70 Valkyrie Fuel Line 270

11.3 Cocaine Case 272

11.4 The Preppy Murder 274

12 Conclusion 282

12.1 Introduction 282

12.2 Solving World Problems 283

12.3 Lifelong Learning 287

12.4 Continued Evolution of Microscopy and Photonics 288

12.5 Final Thoughts 290

Index 292