Single Molecule Techniques

A Laboratory Manual

By: Paul R. Selvin (Editor), Taekjip Ha (Editor)

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Paperback | 19 November 2007 | Edition Number 1

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As molecular and cellular biologists move toward nano-techniques for performing experiments on single molecules rather than on populations of molecules, a comprehensive manual on how (and why) to carry out such experiments is needed. Single-Molecule Techniques: A Laboratory Manual fills this requirement - it is the first to take researchers who know nothing about single-molecule analyses to the point where they can successfully design and execute appropriate experiments. Geared toward research scientists in structural and molecular biology, biochemistry, and biophysics, the manual will be useful to all who are interested in observing, manipulating, and elucidating the molecular mechanisms and discrete properties of macromolecules. Techniques range from in vivo and in vitro fluorescent-based methods to the use of atomic force microscopy, optical and magnetic tweezers, and nanopores. The book is edited by Paul R. Selvin and Taekjip Ha, two pioneers in the field of experimental biophysics who have made significant contributions to the development and application of single-molecule technologies.

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I don't believe I have ever before read a microscopy textbook from cover to cover - but this one, reader, I did. Its two editors, Taekjip Ha and Paul Selvin, have set out to assemble a manual for cell biologists who want to extend their technical repertoires to include single-molecule techniques. They have succeeded admirably, and as a bonus the book provides a fascinating insight into where the technical limits lie, of how we stand - as the authors suggest in their introduction - at the beginning of a new era of single-molecule-based cell biology...This excellent book will be invaluable to anyone who is already competent at multimolecule data collection and interested in getting down to molecular mechanisms. Nature Cell Biology

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Preface
The New Era of Biology In Singulo	p. 1
Single-Molecule FRET with Total Internal Reflection Microscopy	p. 3
In Vitro and In Vivo FIONA and Other Acronyms for Watching Molecular Motors Walk	p. 37
Colocalization of Fluorescent Probes: Accurate and Precise Registration with Nanometer Resolution	p. 73
Alternating-Laser Excitation of Single Molecules	p. 85
Orientation and Rotational Motions of Single Molecules by Polarized Total Internal Reflection Fluorescence Microscopy	p. 121
Imaging Gene Expression in Living Cells at the Single-Molecule Level	p. 149
Single-Virus Tracking in Live Cells	p. 171
Ultrasensitive Imaging in Live Cells Using Fluorescent Quantum Dots	p. 193
Imaging Real-Time Gene Expression in Living Systems	p. 209
Single-Molecule Imaging of Stochastic Signaling Events in Living Cells	p. 239
Fluorescence Correlation Spectroscopy In Vitro and In Vivo	p. 259
Optical Traps to Study Properties of Molecular Motors	p. 279
High-Resolution Dual-Trap Optical Tweezers with Differential Detection	p. 297
Imaging and Nanomanipulation of an Actomyosin Motor	p. 325
Single-Molecule Studies Using Magnetic Traps	p. 347
Probing Polysaccharide and Protein Mechanics by Atomic Force Microscopy	p. 371
Single-Molecule Analysis of Nucleic Acids and DNA-Protein Interactions Using Nanopores	p. 395
Single-Molecule Gold-Nanoparticle Tracking with High Temporal and Spatial Resolution and without an Applied Load	p. 421
Advances in Surface-based Assays for Single Molecules	p. 431
Hydrodynamic Flow-stretching Assay for Single-Molecule Studies of Nucleic Acid-Protein Interactions	p. 461
Cautions	p. 493
Index	p. 501
Table of Contents provided by Blackwell. All Rights Reserved.

Single Molecule Techniques

A Laboratory Manual

At a Glance

Paperback

Industry Reviews

Shipping

How to return your order

Defective items

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