Separations for Biotechnology 2

Plenary Paper.- Frontiers of Bioseparations Technology: Unsolved Problems and Novel Process Concepts.- 1: Cell Disruption and Removal of Insolubles.- Cell Disruption and Removal of Insolubles (Review Paper).- Differential Product Release from Yeast Cells by Selective Enzymatic Lysis.- Conceptual Design of a Novel Flocculation Device.- The Effect of Culture History on the Disruption of Alcaligenes eutrophus by High Pressure Homogenisation.- Separation by Biopolymers: Separation of Suspended Solids by Microbial Flocculant.- Protein Release from the Yeast Pichia pastoris by Chemical Permeabilization: Comparison to Mechanical Disruption and Enzymatic Lysis.- The Concentration of Yeast Suspensions by Crossflow Filtration.- Heterologous Flocculation of Escherichia coli and Yeast.- Lateral Migration: A Laminar Fluid Flow Mechanism Suited to Biotechnology Separations.- The Dewatering of Biological Suspensions in Industrial Centrifuges.- 2: Concentration Processes.- Concentration and Selectivity in Bioseparations (Review Paper).- A Model for Emulsion Liquid Membrane Extraction of Organic Acids.- Vortex Flow Filtration for Ultrafiltration of Protein Solutions.- Membrane Filtration: a priori Predictions of the Ideal Limiting Flux.- Clarification of Tissue Culture Fluid and Cell Lysates Using Biocryl(R) Bioprocessing Aids.- Economic Fabrication and Utilisation of Agar Composites in Bioselective Recovery in Fixed and Fluidised Beds.- Electromagnetic Separation of Small Particles in the Dielectric Regime.- Reactive Solvent Extraction of Beta-Lactam Antibiotics.- Aqueous Two-Phase Fractionation: Practical Evaluation for Productive Biorecovery.- Urease Immobilized on an Acrylic Carrier for Enzymatic Removal of Urea.- Solid Phases for Immunoaffinity Adsorption.- The Relationship between Protein Partition Coefficient and Polymer Concentration in Aqueous Two-Phase Systems.- Protein Fouling and its Implications for Selection of Ultrafiltration Membranes.- Purification by Two-Phase Partitioning of a Hepatitis Core Protein- Pertussis Epitope Fusion, Expressed in Yeast.- Separation of Low-Molecular Organic Compounds from Complex Aqueous Mixtures by Extraction.- Extraction of Citric Acid Using a Supported Liquid Membrane.- 3: High Resolution Separations.- The Future of High Resolution Purification (Review Paper).- Stable Synthetic Affinity Ligands for Use in Protein Fractionation.- The Utility of Polymeric Reversed Phase Packings for the Purification of Peptides, Proteins and Antibiotics.- Batch Stirred Tank Adsorption of Impurity of Cephalosporin C, Desacetylcephalosporin C, Using Modified Amberlite XAD-2 Resin.- Modification of Microfiltration Membranes as Dye-Ligand Adsorbents for the Isolation of Enzymes from Crude Extracts.- Adsorption-Desorption of Proteins in RP-HPLC RNase on C4 Supports.- Affinity Separations Using Microcapsules.- Modelling of Protein Adsorption in Liquid Fluidized Beds.- Affinity Separation of Proteins in Aqueous Two-Phase Systems.- Effect of pH on Polygalacturonase Adsorption in CM-Sephadex Gels.- Purification and Characterisation of Sarcosine Dehydrogenase.- Separation of Some Sulphated Olivanic Acids Using Immobilised Cyclodextrins.- Separation with Porous Glass Membranes and Controlled Pore Glass (CPG) Chromatography.- Performance on Anion-Exchange Cellulose at Process-Scale.- High Speed Analytical and Preparative Separations of Biological Macromolecules.- Purification and Characterisation of Gentamicin Acetyl Transferase.- Affinity Purification Using Radial Streaming Affinity Chromatography.- Multi-component Adsorption of Proteins to Ion Exchangers.- 4: Product Finishing.- The Manufacture of Proteins for Human Therapeutic Use (Review Paper).- Immunoaffinity Purification of Guar Alpha-Galactosidase Using Mild Elution Conditions.- Purification of Mouse IgGl on Protein A and the Measurement of Contaminating Protein A.- Analyses of Purity and Homogeneity in Production Processes.- Purification of Monoclonal Antibodies for Human Clinical Use: Validation of DNA and Retroviral Clearance.- Analysis of Recombinant Human IFN-Gamma Produced in CHO Cells: Effects of Glycosylation and Proteolytic Processing on Product Heterogeneity.- Validation of the Re-use of Protein A Sepharose for the Purification of Monoclonal Antibodies.- Process Design for the Inactivation of Viruses in the Manufacture of Pharmaceutical Proteins.- Investigations of Apparent Homogeneity and Stability of Recombinant HuIFN-Alpha 1.- 5: Integrated Processes.- The Rational Design of Large Scale Protein Separation Processes.- Downstream Processing of Extracellular Enzymes of Aspergillus niger.- Ethanol Production by Extractive Fermentation: A Novel Membrane Extraction Technique.- Continuous Chromatographic Bioreaction-Separation.- Thermodynamic Analysis of Separation Process Alternatives.- Secondary Metabolites by Extractive Fermentation.- Cloning and Expression as a Tool to Easily Purify a New Highly Thermostable Archaebacterial ?-Galactosidase.- 6: Downstream Systems Design and Control.- Downstream Process Design and Bioproduct Quality Control (Review Paper).- Biosafety in Downstream Processing.- Automated Multi-stage Chromatographic Systems for Production of Bio therapeutics.- Applications of Molecular Size Detection in Column Chromatography.- The Monitoring and Control of Protein Purification and Recovery Processes.- A Study of Permeability and Hydrodynamic Dispersion under Conditions of Chromatographic Flow.- Simple Diagnostic Guidelines for Adsorption Column Performance.- Index of Contributors.