PART I: Protocols for preparing primary cultures, cell lines and engineered Schwann cells
1. Isolation of Schwann Cell Precursors from Rodents
Rhona Mirsky and Kristjan R. Jessen
2. Preparation of Neonatal Rat Schwann cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies
Patrice Maurel
3. Isolation and Expansion of Schwann Cells from Transgenic Mouse Models
Jihyun Kim and Haesun Kim
4. Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers
Natalia D. Andersen and Paula V. Monje
5. Detailed Protocols for the Isolation, Culture, Enrichment, and Immunostaining of Primary Human Schwann cells
Tamara Weiss, Sabine Taschner-Mandl, Peter F. Ambros, and Inge M. Ambros
6. Magnetic-activated Cell Sorting for the Fast and Efficient Separation of Human and Rodent Schwann Cells from Mixed Cell Populations
Kristine M. Ravelo, Natalia D. Andersen, and Paula V. Monje
7. Culture and Expansion of Rodent and Porcine Schwann Cell for Pre-clinical Animal Studies
Adriana E. Brooks, Gagani Athauda, Mary Bartlett Bunge, and Aisha Khan
8. Chemical Conversion of Human Fibroblasts into functional Schwann Cells
Eva C. Thoma
9. Derivation of Fate-committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats
Y.P. Tsui, Graham K. Shea, Y.S. Chan, and Daisy K. Shum
10. Human Induced Pluripotent Stem Cell (hiPS cell)-derived Sensory Neurons for Fate Commitment of Bone Marrow Stromal Cell-derived Schwann Cells
Sa Cai, Daisy KY Shum, and Ying-Shing Chan
11. Generation and Use of Merlin-Deficient Human Schwann Cells for a High-Throughput Chemical Genomics Screening Assay
Alejandra M. Petrilli and Cristina Fernandez-Valle
PART II: Protocols for studying Schwann cells behavior and myelination in vitro
12. Lentiviral Transduction of Rat Schwann Cells and Dorsal Root Ganglia Neurons for In Vitro Myelination Studies
Corey Heffernan and Patrice Maurel
13. Preservation, Sectioning and Staining of Schwann Cell Cultures for Transmission Electron Microscopy Analysis
Vania W. Almeida, Margaret L. Bates, and Mary Bartlett Bunge
14. Scalable Differentiation and Dedifferentiation Assays using Neuron-free Schwann Cell Cultures
Paula V. Monje
15. The Pseudopod System for Axon-glia Interactions: Stimulation and Isolation of Schwann cell Protrusions that form in Response to Axonal Membranes
Yannick Poitelon and M. Laura Feltri
16. In vitro Analysis of the Role of Schwann Cells on Axonal Degeneration and Regeneration using Sensory Neurons from Dorsal Root Ganglia
Rodrigo Lopez-Leal, Paula Diaz, and Felipe A. Court
17. A Culture Model To Study Neuron - Schwann cell - Astrocyte Interactions
Susana R. Cerqueira, Yee-Shuan Lee, and Mary Bartlett Bunge
18. Preparation of Matrices of Variable Stiffness for the Study of Mechanotransduction in Schwann Cell Development
Mateusz M. Urbanski and Carmen V. Melendez-Vasquez
19. Purification of Exosomes from Primary Schwann cells, RNA Extraction and Next Generation Sequencing of Exosomal RNAs
Cristian De Gregorio, Paula Diaz, Rodrigo Lopez-Leal, Patricio Manque, and Felipe A. Court
20. 3D Cancer Migration Assay with Schwann Cells
Laura Fangmann, Steffen Teller, Pavel Stupakov, Helmut Friess, Guralp O. Ceyhan, and Ihsan Ekin Demir
PART III: Protocols for studying Schwann cell development and myelination in viv
