Receptor Binding Techniques

1. Receptor Databases and Computational Websites for Ligand Binding Brinda K. Rana, Philip E. Bourne and Paul A. Insel 2. How to use the IUPHAR Receptor Database to Navigate Pharmacological Data Chidochangu P. Mpamhanga, Joanna L. Sharman, Anthony J. Harmar, and NC-IUPHAR 3. Radioligand Binding Assays and their Analysis Janet J. Maguire, Rhoda E. Kuc and Anthony P. Davenport 4. Use of Scintillation Proximity Assay (SPA) to Measure Radioligand Binding to Immobilised Receptors without Separation of Bound from Free Ligand Jenny Berry, Molly Price-Jones, and Barbara Killian 5. Visualisation and Analysis of Vascular Receptors using Confocal Laser Scanning Microscopy and Fluorescent Ligands Craig J Daly, Ingela Parmryd and John C. McGrath 6. Dissecting the Pharmacology of G Protein Coupled Receptor Signalling Complexes using Bimolecular Fluorescence Complementation Laura E Kilpatrick and Nicholas D Holliday 7. Live Cell Imaging of G Protein-Coupled Receptors Anke Teichmann, Antje Schmidt , Burkhard Wiesner , Alexander Oksche , and Ralf Schulein8. Characterization of G-protein Coupled Receptor (GPCR) Modulators using Homogeneous cAMP Assays Daniel L. Bassoni, Qumber Jafri, Sunitha Sastry, Mahesh Mathrubutham and Tom Wehrman 9. Measurements of I -arrestin Recruitment to Activated Seven Transmembrane Receptors using Enzyme Complementation Daniel L. Bassoni, William J. Raab, Philip L. Achacoso, Chin Yee Loh and Tom S. Wehrman 10. Quantitative Phosphor Imaging Autoradiography of Radioligands for Positron Emission Tomography Peter Johnstrom, Joseph L. Bird and Anthony P. Davenport 11. Dynamic In Vivo Imaging of Receptors in Small Animals using Positron Emission Tomography Peter Johnstrom, Tim D. Fryer, Joseph L. E. Bird, Hugh K. Richards, and Anthony P. Davenport 12. Cellular Localization of Receptors using Antibodies Visualised by Light and Dual Labelling Confocal Microscopy Anthony P. Davenport and Rhoda E. Kuc 13. Detection of mRNA Encoding Receptors by in Situ And Northern Hybridisation Alessandra P. Princivalle, Rachel M.C Parker, Terri J. Dover, and Nicholas M. Barnes