Practical Exercises in Parasitology

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Paperback | 3 November 2005

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Parasitology is an immensely important aspect of biological science. This manual presents fifty easy-to-follow laboratory exercises for student practical (lab) classes. All the exercises are tried and tested by the authors and are used in a wide variety of university undergraduate teaching departments. They range from relatively simple observational exercises, using local materials and requiring little in the way of equipment, to more technically demanding experiments in physiology and molecular parasitology. Each exercise includes a list of necessary equipment, consumables and sources of parasite material, instructions for staff and students, including aspects of safety, expected results, and some analysis provided by questions. In addition, the text also includes ideas for further exploration and information on similar exercises, as well as lists of selected further reading. This book should be an essential purchase for all teachers of parasitology at the university undergraduate level and for students taking laboratory practical classes in the subject.

Industry Reviews

'The book is an excellent stimulation for lecturers to include such parasitological exercises into their courses.' Zoologischer Anzeiger '... an extremely useful aid.' Oleg Ditrich, Folia Parasitologica 'Edited by experienced teacher/researchers Practical Exercises in Parasitology seems to have thought of everything ... the degree of detail and ancillary information is exceptional ...' Phil Whitfield, Journal of Parasitology '... very useful for undergraduate students to illustrate many of the basic principles of parasitology.' Linda Basson, African Zoology

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Observational Exercises on Parasites: A. Local and domestic hosts as sources of parasites
Parasites of the earthworm: Monocystis (Protozoa) and Rhabditis (Nematoda)
Parasites of marine molluscs (Littorina)
Parasites of fish
Whiting and Diclidophora merlangi (Monogenea)
Parasites of fish
Plaice/flounder and Lepeophtheirus pectoralis (Copepoda)
Parasites of domestic livestock
Pig and Ascaris suum (Nematoda)
Parasites of domestic livestock
Sheep and Fasciola hepatica (Trematoda)
Parasites of crops: Potato Cyst Nematode (PCN) - Globodera pallida (Nematoda)
Laboratory maintained species
Protozoan parasites of the intestinal tract of the cockroach, Periplaneta Americana
Protozoan parasites of the mouse intestinal tract
Rodent malaria
Malaria: an example of a vector transmitted parasite
Larval and adult Echinostoma spp. (Trematoda)
Schistosoma mansoni (Trematoda)
Hymenolepsis diminuta (Cestoda)
Heligmosomoides polygyrus (Nematoda)
Ecology
Pinworms (Nematoda, Oxyuroidea) in the American cockroach, Periplaneta Americana
Distribution and microhabitat of a monogenean on the gills of mackerel
Population dynamics of Gyrodactylus on stickleback
Intraspecific competition in the cestode, Hymenolepsis diminuta in rats
Transmission dynamics and the pattern of dispersion of the cestode, Hymenolepsis diminuta, in the intermediate host population
Physiology and Biochemistry
Hatching in vitro of oncospheres/hexacanth larvae of Hymenolepsis diminuta
Activation of the cysticercoids of Hymenolepsis spp. in vitro
Membrane transport in the cestode, Hymenolepsis diminuta, in vitro
Glycogen utilization and deposition in flatworm parasites
Effects of classical transmitters on the motility of parasite roundworms and flatworms
Electrophysiology of Ascaris suum body muscle
Immunocytochemical demontration of neuroactive substances in helminth parasites
Pathology and Immunology
Encapsulation of foreign matter (not self) by earthworms
Opsonisation of trypanosomes
Production and screening of monoclonal antibodies against Leishmania promastigotes
Pathological effects of Mesocestoides corti and Schistosoma mansoni
Quantification of lymphocyte populations in the spleen and thymus
Use of basic indirect ELISA for the detection of antibodies produced by experimental immunisation
SDS PAGE and Western blotting for the detection of antibodies produced by experimental immunisation
Chemotherapy
Sensitivity of a coccidial parasite, Eimeria, to an ionophore, monensin
Egg hatch assay for determination of resistance of nematodes to benzimidazole anthelmintics
Larval migration inhibition assay for determination of susceptibility of nematodes to levamisole
Effect of anthelmintics on nematodes
Molecular Parasitology
Purification of DNA
DNA digestion and gel electrophoresis
Restriction enzyme mapping
Construction of a genomic library
Detection and differentiation of Entamoeba histolytica and E. dispar by PCR
Differentiation between parasite species by agglutination and detection of parasite surface cardohydrate, using non-conjugated lectins
Tentative identification of parasite and tissue surface carbohydrates by conjugated lectins
Behaviour
Behaviour of the miracidia of Fasciola hepatica and demonstration of other larval stages
Effects of age and environmental factors on the swimming behaviour of the cercariae of Cryptocotyle lingua (Trematoda)
Changes in host behaviour as a consequence of parasite infection
Behaviour of the amphipod, Gammarus pulex, infected with cystacanths of acanthocephalans
Effects of Schistocephalus solidus (Cestoda) on stickleback feeding behaviour
Table of Contents provided by Publisher. All Rights Reserved.

Practical Exercises in Parasitology

At a Glance

Paperback

Industry Reviews

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