Methods in Molecular Biology

This detailed volume explores methods and protocols employed to investigate the multifaceted processes involved in plant stress memory. Opening with foundational methods for studying thermomemory in Arabidopsis thaliana, the book continues with techniques for studying memory mechanisms in other plant species, memory of biotic stressors, and the critical role of signaling molecules, such as jasmonic acid and salicylic acid. A key focus of this book is the investigation of DNA methylation and other epigenetic modifications that play a central role in the transmission of stress memory across generations, and epigenetic and transcriptomic approaches plus cutting-edge techniques such as RNA-Seq, CRISPR/Cas9-based gene editing, and proteomic analyses are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Stress Memory: Methods and Protocols serves as an invaluable resource for researchers, students, and practitioners working to unravel the mysteries of plant stress memory and apply this knowledge to address global challenges in agriculture and environmental sustainability.