Contents Preface Contributors Part I Fixation, Processing, Embedding and Staining of Botanical Specimens 1. A Guide to the Study of Plant Structure with Emphasis on Living Specimens Edward C. Yeung 2. Chemical and Physical Fixation of Cells and Tissues: an Overview Bing Q. Huang and Edward C. Yeung 3. Paraffin and Polyester Waxes Claudio Stasolla and Edward C. Yeung 4. The Glycol Methacrylate Embedding Resins - Technovit 7100 and 8100 Edward C. Yeung and Colin K. W. Chan 5. Epoxy Resins for Light and Transmission Electron Microscopy Michael J. Sumner 6. LR White Acrylic Resin Edward C. Yeung and Bing Q. Huang 7. High Pressure Freezing and Freeze Substitution of In vivo and In vitro Cultured Plant Samples Jose M. Segui-Simarro 8. Three-Dimensional Imaging for Electron Microscopy of Plastic-embedded Plant Specimens Jose M. Segui-Simarro 9. Fluorescent Staining of Living Plant Cells Sarah Schoor, Shiu-Cheung Lung, Dustin Sigurdson, and Simon D. X. Chuong 10. Improved Methods for Clearing and Staining of Plant Samples Alexander Lux, Marek Vaculik, and Jan Kovac Part II Microtechniques related to Cell and Molecular biology studies 11. Whole Mount Immunofluorescence Staining of Plant Cells and Tissues Masaki Shimamura 12. Protoplast Isolation and Staining Shiu-Cheung Lung, Sarah Schoor, Dustin Sigurdson, Makoto Yanagisawa, Kelly Yeung, Mi Qi Liu and Simon D. X. Chuong 13. Guiding Principles for Live Cell Imaging of Plants using Confocal Microscopy Subramanian Sankaranarayanan and Marcus A. Samuel 14. Immunogold Labeling for Electron Microscopy: Strategy and Problem Solving Fengli Guo and Bing Q. Huang 15. Abscisic Acid Immunostaining Yung-I Lee and Mei-Chu Chung 16. Plant Chromosome Preparations and Staining for Light Microscopic Studies Subhash C. Hiremath and C.C. Chinnappa 17. Chromosome techniques and FISH Mei-Chu Chung 18. Detection of S-phase of Cell Division Cycle in Plant Cells and Tissues by using 5-ethynyl-2'-deoxyuridine (EdU) Soujanya Kuntam and Ferhan Ayaydin 19. Staining Methods for Programmed Cell Death Owen S. D. Wally and Claudio Stasolla 20. Laser Microdissection of Plant Tissues Jenna L. Millar, Michael G. Becker, and Mark F. Belmonte 21. RNA In situ Hybridization Shalini Mukherjee, Claudio Stasolla, and Belay Ayele Part III Preparative Methods for the Study of Wood Anatomy 22. Microscopic Examination of Wood: Sample Preparation and Techniques for Light Microscopy Jacques C. Tardif and France Conciatori 23. Collecting and Processing Wood Microcores for Monitoring Xylogenesis Annie Deslauriers, Sergio Rossi, and Eryuan Liang 24. Three-dimensional Imaging of Cambium and Secondary Xylem Cells by Confocal Laser Scanning Microscopy Satoshi Nakaba, Peter Kitin, Yusuke Yamagishi, Shahanara Begum, Kayo Kudo, Widyanto Dwi Nugroho, and Ryo Funada Part IV Botanical Techniques and Protocols for Archeology and Herbarium Collection 25. Collection of Plant Remains from Archaeological Contexts Alessandra Celant, Donatella Magri, and Francesca Romana Stasolla 26. Archaeological Wood Preparation Alessandra Celant and Gemma Coccolini 27. Archaeopalynological Preparation Techniques Donatella Magri and Federico Di Rita 28. Phytoliths: Preparation and Archaeological Extraction Brian Kooyman 29. Starch Granules: Preparation and Archaeological Extraction Brian Kooyman 30. Plant Collection, Identification, and Herbarium Procedures Bonnie Smith and C. C. Chinnappa Index
