My Wish Lists Login / Join

Optimal High-Throughput Screening

Practical Experimental Design and Data Analysis for Genome-Scale RNAi Research

By: Xiaohua Douglas Zhang

Write A Review

Paperback | 21 February 2011

At a Glance

Paperback
238 Pages

Dimensions(cm)
25.4 x 17.7 x 1.3

Paperback

RRP $59.95

$58.75

or 4 interest-free payments of $14.69 with

Ships in 5 to 7 business days

This concise, self-contained and cohesive book focuses on commonly used and recently developed methods for designing and analyzing high-throughput screening (HTS) experiments from a statistically sound basis. Combining ideas from biology, computing and statistics, the author explains experimental designs and analytic methods that are amenable to rigorous analysis and interpretation of RNAi HTS experiments. The opening chapters are carefully presented to be accessible both to biologists with training only in basic statistics and to computational scientists and statisticians with basic biological knowledge. Biologists will see how new experiment designs and rudimentary data-handling strategies for RNAi HTS experiments can improve their results, whereas analysts will learn how to apply recently developed statistical methods to interpret HTS experiments.

Shipping

	Standard Shipping	Express Shipping
Metro postcodes:	$9.99	$14.95
Regional postcodes:	$9.99	$14.95
Rural postcodes:	$9.99	$14.95

Orders over $79.00 qualify for free shipping.

How to return your order

At Booktopia, we offer hassle-free returns in accordance with our returns policy. If you wish to return an item, please get in touch with Booktopia Customer Care.

Additional postage charges may be applicable.

Defective items

If there is a problem with any of the items received for your order then the Booktopia Customer Care team is ready to assist you.

For more info please visit our Help Centre.

You Can Find This Book In

Non-Fiction Medicine Medicine in General Public Health & Preventive Medicine Epidemiology & Medical Statistics Nursing & Ancillary Services Pharmacy & Dispensing Science Biology, Life Sciences Life Sciences in General Genetics excluding Medical DNA & Genome

Mathematics Probability & Statistics Engineering & Technology Biochemical Engineering Biotechnology Applied Mathematics Mathematical Modelling

Preface	p. ix
Acknowledgments	p. xv
Acronyms and Abbreviations	p. xvii
RNAi HTS and Data Analysis
Introduction to Genome-Scale RNAi Research	p. 3
RNAi: An Effective Tool for Elucidating Gene Functions and a New Class of Drugs	p. 3
High-Throughput Screening: A Vital Technology in Drug Discovery	p. 5
Genome-Scale RNAi Screens	p. 7
An Example of Genome-Scale RNAi Research	p. 9
Challenges in Genome-Scale RNAi Research	p. 10
Experimental Designs	p. 13
siRNA Designs	p. 13
Control Designs	p. 15
Plate Designs	p. 16
Designs of siRNA Delivery and Optimization of Transfection	p. 24
Design of Sample Size	p. 24
Conclusions	p. 25
Data Display and Normalization	p. 27
Data Display Using Graphics	p. 27
Transformation of Measured Raw Values	p. 32
Identification and Adjustment of Systematic Spatial Effects	p. 33
Strategy for Data Display and Normalization	p. 41
Quality Control in Genome-Scale RNAi Screens	p. 42
Introduction	p. 42
Quality Assessment Metrics	p. 42
Quality Control Criteria	p. 47
Adoption of Effective Plate Designs	p. 53
Integration of Experimental and Analytic Approaches to Improve Data Quality	p. 56
Application	p. 57
Discussion and Conclusions	p. 60
Hit Selection in Genome-Scale RNAi Screens without Replicates	p. 62
Introduction	p. 62
Methods for Hit Selection in Primary Screens without Replicates	p. 63
Decision Rules for Hit Selection in RNAi Screens	p. 69
Sample Size Determination	p. 74
Applications	p. 77
Conclusions	p. 82
Hit Selection in Genome-Scale RNAi Screens with Replicates	p. 83
Metrics for Hit Selection in Screens with Replicates	p. 83
Dual-Flashlight Plot	p. 86
Decision Rules for Hit Selection in Screens with Replicates	p. 88
False Discovery Rate, False Non-Discovery Rate, q-Value, and q^* -Value	p. 90
Sample Size Determination	p. 92
Analytic Methods Adjusting for Off-Target Effects	p. 93
Applications	p. 98
Discussion and Conclusions	p. 106
Methodological Development for Analyzing RNAi HTS Screens
Statistical Methods for Group Comparison	p. 111
Illustration of Issues in Traditional Contrast Analysis	p. 112
Contrast Variable, SMCV, and c⁺-Probability	p. 114
A Classifying Rule for Interpreting Strength of Group Comparisons	p. 116
A Theorem to Facilitate the Estimation and Inference of SMCV	p. 119
Estimation of SMCV and c⁺-Probability	p. 127
Contrasts in Multifactor ANOVA	p. 133
Case Studies and Simulation	p. 142
Discussion and Conclusions	p. 152
Statistical Methods for Assessing the Size of siRNA Effects	p. 154
SSMD and d⁺-Probability	p. 154
Estimation of SSMD	p. 156
Comparing SSMD with Standardized Mean Difference and Classical t-Statistic	p. 159
SSMD-Based Ranking Methods for Hit Selection in Genome-Scale RNAi Screens	p. 165
SSMD-Based FPR, FNR, and Power	p. 166
FDR and FNDR in RNAi Screens	p. 176
Analytic Methods Adjusting for Off-Target Effects	p. 179
Discussion and Conclusions	p. 186
References	p. 189
Index	p. 201
Table of Contents provided by Ingram. All Rights Reserved.

Optimal High-Throughput Screening

Practical Experimental Design and Data Analysis for Genome-Scale RNAi Research

At a Glance

Paperback

Shipping

How to return your order

Defective items

You Can Find This Book In

More in Applied Mathematics

AI for Decision Intelligence in Critical Systems

Advances in Applied Mathematics

AI for Decision Intelligence in Critical Systems

Advances in Applied Mathematics

Dynamics of Marine Structures

Stochastic Control in Energy, Environmental and Resource Management Applications

Emerging Methodologies and Applications in Modelling, Identification and Control

Mathematics of Uncertainty Applied to Global Issues

Sustainability, Mistreatment of Women, Nuclear Power, Smart Cities, Digitalization

Applied Multivariate Statistical Analysis

Volume 1

Advances in Numerical Methods and Analysis

The Gamma Function (Advanced Topics in Mathematics)

Advanced Topics in Mathematics

Storytelling with Data

1st Edition - A Data Visualization Guide for Business Professionals

Bird's Engineering Mathematics

9th Edition

The Maths Book

Big Ideas Simply Explained

Essentials of Medical Statistics

2nd Edition

A Mind for Numbers

How to Excel at Math and Science (Even If You Flunked Algebra)

Engineering Mathematics

5th edition

Bird's Higher Engineering Mathematics

9th Edition

Game Theory

2nd edition

Advanced Engineering Mathematics, International Adaptation

Storytelling with Data

A Data Visualization Guide for Business Professionals, 10th Anniversary Edition

Intermediate Financial Theory

3rd Edition

Introduction to Stochastic Processes

2nd Edition

An Introduction To Quantum Field Theory

MATLAB

6th Edition - A Practical Introduction to Programming and Problem Solving

Swim Training Patterns

1st Edition - Plan your Training Sessions with the Power of Mathematics

Elementary Linear Algebra

8th Edition - International Metric Edition

This product is categorised by