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Methods in Molecular Biology

Cellular and Molecular Methods

By: Kerry S. Campbell (Editor)

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Hardcover | 17 December 2009 | Edition Number 2

At a Glance

Hardcover
566 Pages

Edition Type
Revised

Dimensions(cm)
26.04 x 18.42 x 3.81

Hardcover

$379.01

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As the research has continued, it has become increasingly clear that natural killer (NK) cells are critical sentinels of the innate immune response, playing important roles in protecting the body from numerous pathogens and cancer in addition to contributing to normal pregnancy and impacting the outcomes of transplantation. While the first edition provided a valuable collection of classical cellular and in vivo techniques to study NK cell functions, the Second Edition of "Natural Killer Cell Protocols: Cellular and Molecular Methods" brings together more recently developed methods, more refined techniques, and detailed protocols designed to study NK cells within specialized tissue sites in both mice and humans. In this collection of methods, international leaders in the field cover topics ranging from the analysis of the various stages of NK cell development and maturation to specialized techniques for the identification of ligands for NK cell receptors. This volume also includes an appendix, providing a rich resource summarizing available reagents to study NK cells, cross-referencing KIR nomenclature, and detailing the many HLA ligands for various KIR family members. As a volume in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and thorough notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, "Natural Killer Cell Protocols: Cellular and Molecular Methods, Second Edition" seeks to aid researchers and further advance our understanding of the functions, maturation, and regulation of these fascinating and dynamic cells.

Industry Reviews

From the reviews of the second edition:

"Natural Killer Cell Protocols is a comprehensive collection of in vivo and in vitro protocols edited by Kerry S. Campbell. ... designed for those who have specific questions to ask about NK cells and to answer such questions through a detailed experimental plan using the most up-to-date techniques. ... this book may indeed fulfil its remit in offering hands-on protocols to solve problems of day to day investigation for NK-cell biologists. Good value if you can persuade your lab-head or librarian to invest." (Anna Ettorre and Danny Altmann, Immunology News, November, 2010)

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You Can Find This Book In

Non-Fiction Medicine Clinical & Internal Medicine Diseases & Disorders Immunology Science Biology, Life Sciences Molecular Biology Cellular Biology Pre-Clinical Medicine & Basic Sciences Science in General Scientific Equipment

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Paperback

Published: 23rd August 2016

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Preface	p. v
Contributors	p. xi
Purification of Human NK Cell Developmental Intermediates from Lymph Nodes and Tonsils	p. 1
In Vitro Development of Human Killer-Immunoglobulin Receptor-Positive NK Cells	p. 15
Subset Analysis of Human and Mouse Mature NK Cells	p. 27
Assessing Licensing of NK Cells	p. 39
Use of Stem Cell Radiation Chimeras to Analyze How Domains of Specific Proteins Impact on Murine NK Cell Development In Vivo	p. 51
Use of Transfected Drosophila S2 Cells to Study NK Cell Activation	p. 67
Natural Killer Cell Conjugate Assay Using Two-Color Flow Cytometry	p. 89
Studying NK Cell/Dendritic Cell Interactions	p. 97
Analysis of the NK Cell Immunological Synapse	p. 127
Measuring Intracellular Calcium Signaling in Murine NK Cells by Flow Cytometry	p. 149
Intracellular Staining for Analysis of the Expression and Phosphorylation of Signal Transducers and Activators of Transcription (STATs) in NK Cells	p. 159
A Model System for Studying NK Cell Receptor Signaling	p. 177
Expression of cDNAs in Human Natural Killer Cell Lines by Retroviral Transduction	p. 199
Lentiviral Gene Transduction in Human and Mouse NK Cell Lines	p. 209
Introduction of shRNAs into Human NK-Like Cell Lines with Retrovirus	p. 223
Introduction of shRNAs into Primary NK Cells with Lentivirus	p. 233
Methods to Identify and Characterize Different NK Cell Receptors and Their Ligands	p. 249
Generating NK Cell Receptor-Fc Chimera Proteins from 293T Cells and Considerations of Appropriate Glycosylation	p. 275
Identification of NK Cell Receptor Ligands Using a Signaling Reporter System	p. 285
Determining Ligand Specificity of Ly49 Receptors	p. 299
Probing the Interactions of NK Cell Receptors with Ligand Expressed in trans and cis	p. 313
A Simple Method to Measure NK Cell Cytotoxicity In Vivo	p. 325
Functional Analysis of Human NK Cells by Flow Cytometry	p. 335
Analysis of the KIR Repertoire in Human NK Cells by Flow Cytometry	p. 353
KIR Genotyping by Multiplex PCR-SSP	p. 365
Identification and Analysis of Novel Transcripts and Promoters in the Human Killer Cell Immunoglobulin-like Receptor (KIR) Genes	p. 377
Use of Inbred Mouse Strains to Map Recognition Receptors of MCMV Infected Cells in the NK Cell Gene Locus	p. 393
Studying NK Cell Responses to Ectromelia Virus Infections in Mice	p. 411
Activation of Human NK Cells by Malaria-Infected Red Blood Cells	p. 429
Natural Killer Cells in Human Pregnancy	p. 447
Analysis of Uterine Natural Killer Cells in Mice	p. 465
Isolation of NK Cells and NK-Like Cells from the Intestinal Lamina Propria	p. 505
Appendix	p. 519
Index	p. 545
Table of Contents provided by Ingram. All Rights Reserved.

Methods in Molecular Biology

Cellular and Molecular Methods

At a Glance

Hardcover

Industry Reviews

Shipping

How to return your order

Defective items

You Can Find This Book In

Other Editions and Formats

Paperback

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