Molecular Biology Techniques

An Intensive Laboratory Course

By: Walt Ream, Katherine G. Field

Write A Review

Paperback | 12 November 1998

At a Glance

Paperback
248 Pages

Dimensions(cm)
24.41 x 16.99 x 1.32

Paperback

$234.99

or 4 interest-free payments of $58.75 with

or

Ships in 10 to 15 business days

This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression.

Provides well-tested experimental protocols for each technique
Lists the reagents and preparation of each experiment separately
Contains a complete schedule of experiments and the preparation required
Includes study questions at the end of each chapter

Shipping

	Standard Shipping	Express Shipping
Metro postcodes:	$9.99	$14.95
Regional postcodes:	$9.99	$14.95
Rural postcodes:	$9.99	$14.95

Orders over $79.00 qualify for free shipping.

How to return your order

At Booktopia, we offer hassle-free returns in accordance with our returns policy. If you wish to return an item, please get in touch with Booktopia Customer Care.

Additional postage charges may be applicable.

Defective items

If there is a problem with any of the items received for your order then the Booktopia Customer Care team is ready to assist you.

For more info please visit our Help Centre.

More in Molecular Biology

Togetherness

Symbiosis and the Hidden Story of Life's Greatest Collaborations

Paperback

RRP $36.99

$29.75

20%
OFF

The Body Electric : Electromagnetism and the Foundation of Life - Gary Selden

$141.75

14%
OFF

Molecular Cell Biology (9th Edition) - Harvey Lodish

$116.75

23%
OFF

Molecular Biology : Principles and Practice (2nd Edition) - Michael Cox

$77.99

15%
OFF

Human Genetics

13th Edition

Paperback

RRP $169.95

$155.75

ISE Prescott's Principles of Microbiology

2nd Edition

Paperback

RRP $159.95

$139.75

13%
OFF

Parasitology

2nd Edition - A Conceptual Approach

Paperback

RRP $221.00

$199.75

10%
OFF

Human Osteology, Third Edition

Hardcover

RRP $170.95

$133.99

22%
OFF

Structural Study of Amyloid-×ý

Methods in Enzymology

Hardcover

RRP $390.95

$345.99

12%
OFF

Botany

7th Edition - An Introduction to Plant Biology

Paperback

RRP $427.75

$266.75

38%
OFF

Preface
Course Synopsis: Introduction
Safety Precautions
Daily Schedule
Acknowledgements
Exercises: I DNA Preparation, Polymerase Chain Reaction, and Molecular Cloning: Cesium chloride-ethidium bromide density gradient centrifugation
PCR to synthesize virD2 flanked with restriction sites
Restriction digests of plasmid pGEX2 and PCR products
Purification of DNA fragments from agarose
Ligation of PCR product to pGEX2 vector
Transformation of E. coli with the ligated plasmid
Small-scale preparation of plasmid DNA by the alkaline lysis method
Restriction analysis
Study questions
Protein Expression, Purification, and Analysis: Expression and purification of a fusion protein
SDS-polyacrylamide gel electrophoresis
Silver stain detection of proteins
Study questions
Oligonucleotide-Directed Mutagenesis: Restriction digests of virD2 (in pCS64) and pUC119
Purification of DNA fragments from agarose
Ligatation of restriction fragment and vector
Transformation of E. coli with the ligated plasmid and recovery clones
Small-scale preparation of plasmid DNA from broth cultures
Restriction digest of DNA: examination to confirm insert
Preparation of single-stranded DNA template
Phosphorylation of oligonucleotide
Annealing mutant oligonuleotide to template
In vitro DNA synthesis by primer extension
Transform synthesis reaction into E. coli DH5a
Small-scale preparation of plasmid DNA
Confirmation of mutants by restriction analysis
Study questions
DNA Sequencing: Polyacrylamide sequencing gel electrophoresis
Didexy sequencing
Automated Sequencing
Introduction to databases and gene sequence analysis
Study questions
Southern Blot Detection of DNA: Preparation of genomic DNA from Agrobacterium tumefaciens
Restriction digestion of genomic DNA
Agarose gel electrophoresis of restriction fragments
Southern blot: denaturation and blotting of DNA gel
Preparation of probe nick translation
Hybridization and washing of Southern blots
Study questions
Northern Blot Detection of mRNA: Preparation of RNA from tobacco leaves
Agarose-formaldehyde gel electrophoresis
Northern blot: denaturation and blotting of RNA
Probe preparation
Hybridization and washing of Northern blots
Study questions
Protein Interaction Analysis in Yeast: Yeast transformation
Filter b-galactosidase assay
Index
Table of Contents provided by Publisher. All Rights Reserved.

Molecular Biology Techniques

An Intensive Laboratory Course

At a Glance

Paperback

Shipping

How to return your order

Defective items

You Can Find This Book In

More in Molecular Biology

Togetherness

Symbiosis and the Hidden Story of Life's Greatest Collaborations

The Body Electric

Electromagnetism and the Foundation of Life