Methods in Molecular Biology

Signalling Nucleic Acid Probes, Methods, and Protocols

By: Andreas Marx (Editor), Oliver Seitz (Editor)

Write A Review

Hardcover | 2 May 2012

At a Glance

Hardcover
280 Pages

Dimensions(cm)
22.86 x 15.88 x 1.91

Hardcover

$169.00

or 4 interest-free payments of $42.25 with

or

Ships in 5 to 7 business days

Significant DNA sequence data has been made available by several important genome projects. The allocation of functions to a mere string of the four letters in the code of life plays a major if not predominant role of ongoing and future research. Guided by experts in the field, Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols, provides easy-to-follow experiments, commences with an introduction to the basic principles of fluorescence, and shows a diverse set of instructive examples, from probe design to applications in clinical settings, molecular beacon technology, the identification of transgenic mice, along with the potential of realtime PCR. While originally being used for fluorogenic nucleic acid probes with a stem-loop structure, molecular beacon now refers to a whole family of fluorescence-up probes. Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols summarizes all the latest findings and explores powerful techniques designed for geneticist, biotechnologists, molecular and cellular biologists, researchers and developers.

Shipping

	Standard Shipping	Express Shipping
Metro postcodes:	$9.99	$14.95
Regional postcodes:	$9.99	$14.95
Rural postcodes:	$9.99	$14.95

Orders over $89.00 qualify for free shipping.

How to return your order

At Booktopia, we offer hassle-free returns in accordance with our returns policy. If you wish to return an item, please get in touch with Booktopia Customer Care.

Additional postage charges may be applicable.

Defective items

If there is a problem with any of the items received for your order then the Booktopia Customer Care team is ready to assist you.

For more info please visit our Help Centre.

More in Genetics excluding Medical

Lifespan

Why We Age - and Why We Don't Have To

Paperback

RRP $26.99

$22.99

15%
OFF

The Selfish Gene

40th Anniversary Edition

Paperback

RRP $32.95

$26.75

19%
OFF

The Genetic Book of the Dead

A Darwinian Reverie

Paperback

RRP $24.99

$19.75

21%
OFF

Biology of Belief

Paperback

RRP $32.99

$26.99

18%
OFF

Genetic and Epigenetic Damage in Human Spermatozoa : Advances in Experimental Medicine and Biology - Ettore Caroppo

$29.75

20%
OFF

Polyploid Plants : Recent Trends and Future Perspectives - John Martin

$21.75

13%
OFF

Every Living Thing

The Great and Deadly Race to Know All Life (WINNER OF THE 2025 PULITZER PRIZE FOR BIOGRAPHY)

Paperback

RRP $24.99

$21.75

13%
OFF

Genetics For Dummies

4th Edition

Paperback

RRP $41.95

$33.75

20%
OFF

Fundamentals of Forensic DNA Typing

Paperback

RRP $85.95

$63.75

26%
OFF

Dr. Gundry's Diet Evolution

Turn Off the Genes That Are Killing You and Your Waistline

Paperback

RRP $35.00

$28.75

18%
OFF

Human Genetics

13th Edition

Paperback

RRP $169.95

$152.75

10%
OFF

Concepts of Genetics

12th Global Edition

Paperback

RRP $145.90

$111.75

23%
OFF

Genetics

8th Edition - From Genes To Genomes ISE

Paperback

RRP $159.95

$138.75

13%
OFF

Emery's Elements of Medical Genetics and Genomics

16th Edition

Paperback

RRP $107.95

$78.75

27%
OFF

Genetics and Genomics in Medicine

2nd Edition

Paperback

RRP $148.75

$93.99

37%
OFF

Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology

8th edition

Paperback

RRP $91.95

$77.99

15%
OFF

Equine Clinical Medicine, Surgery and Reproduction

2nd edition

Hardcover

RRP $494.00

$327.75

34%
OFF

Thompson & Thompson Genetics in Medicine : 9th Edition

9th Edition

Paperback

RRP $119.95

$86.75

28%
OFF

Preface	p. v
Contributors	p. xi
Introduction
Basic Principles of Fluorescence and Energy Transfer	p. 3
Applications
Multiplex Detection of Mutations	p. 23
Detection of Salmonella Species in Foodstuffs	p. 33
Identification of Homozygous Transgenic Mice by Genomic Real-Time PCR	p. 45
Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma	p. 59
Absolute Quantitation of Feline Leukemia Virus Proviral DNA and Viral RNA Loads by TaqMan Real-Time PCR and RT-PCR	p. 73
High-Throughput Real-Time PCR	p. 89
Design and Use of Scorpions Fluorescent Signalling Molecules	p. 99
Hybridization Probe Pairs and Single-Labeled Probes: an Alternative Approach for Genotyping and Quantification	p. 117
New Technologies
EasyBeacons for the Detection of Methylation Status of Single CpG Duplets	p. 137
Quenched Autoligation Probes	p. 161
HyBeacon Probes for Rapid DNA Sequence Detection and Allele Discrimination	p. 171
FIT-Probes in Real-Time PCR	p. 187
SNP Genotyping by Unlabeled Probe Melting Analysis	p. 199
Non-Nucleic Acid Targets
Molecular Beacons for Protein-DNA Interaction Studies	p. 209
DNA Polymerase Profiling	p. 225
Modular Reporter Hairpin Ribozymes for Analyzing Molecular Interactions	p. 237
Screening of Molecular Interactions Using Reporter Hammerhead Ribozymes	p. 251
Index	p. 265
Table of Contents provided by Ingram. All Rights Reserved.

Methods in Molecular Biology

Signalling Nucleic Acid Probes, Methods, and Protocols

At a Glance

Hardcover

Shipping

How to return your order

Defective items

You Can Find This Book In

Other Editions and Formats

Paperback

More in Genetics excluding Medical

Lifespan

Why We Age - and Why We Don't Have To

The Selfish Gene

40th Anniversary Edition

The Genetic Book of the Dead

A Darwinian Reverie

Biology of Belief

Genetic and Epigenetic Damage in Human Spermatozoa

Advances in Experimental Medicine and Biology

Retinal Gene Therapy

Methods and Protocols

The Knowledge Gene

The incredible story of the supergene that gives us human creativity

Polyploid Plants

Recent Trends and Future Perspectives

Carcinogens, DNA Damage and Cancer Risk

Unlock Ideas

Genome-Wide Association Studies and Genomic Prediction in Plants

Crypt

Life, Death and Disease in the Middle Ages and Beyond

Every Living Thing

The Great and Deadly Race to Know All Life (WINNER OF THE 2025 PULITZER PRIZE FOR BIOGRAPHY)

Genetics For Dummies

4th Edition

Fundamentals of Forensic DNA Typing

Dr. Gundry's Diet Evolution

Turn Off the Genes That Are Killing You and Your Waistline

Human Genetics

13th Edition

Concepts of Genetics

12th Global Edition

Genetics

8th Edition - From Genes To Genomes ISE

Emery's Elements of Medical Genetics and Genomics

16th Edition

Genetics and Genomics in Medicine

2nd Edition

Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology

8th edition

Equine Clinical Medicine, Surgery and Reproduction

2nd edition

Thompson & Thompson Genetics in Medicine : 9th Edition

9th Edition

This product is categorised by