Preface...Table of Contents...Contributing Authors...1. Overview of Mitochondrial BioenergeticsVitor M.C. Madeira2. Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal CellsAna M. Silva and Paulo J. Oliveira3. High-Resolution FluoRespirometry and OXPHOS Protocols for Human Cells, Permeabilized Fibres from Small Biopsies of Muscle and Isolated MitochondriaCarolina Doerrier, Luiz F. Garcia-Souza, Gerhard Krumschnabel, Yvonne Wohlfarter, Andras T. Meszaros, and Erich Gnaiger4. High-Throughput Analysis of Mitochondrial Oxygen ConsumptionJames Hynes, Rachel L. Swiss, and Yvonne Will5. Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal SlicesAna Ledo, Rui M. Barbosa, and Joao Laranjinha6. Mitochondrial Membrane Potential (ΠΨ) Fluctuations Associated with the Metabolic States of MitochondriaJoao S. Teodoro, Anabela P. Rolo, and Carlos M. Palmeira7. Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured CellsDavid G. Nicholls8. Comparison of Mitochondrial Incubation Media for Measurement of Respiration and Hydrogen Peroxide ProductionTimea Komlodi, Ondrej Sobotka, Gerhard Krumschnabel, Nicole Bezuidenhout, Elisabeth Hiller, Carolina Doerrier, and Erich Gnaiger9. Measurement of Proton Leak in Isolated MitochondriaCharles Affourtit, Hoi-Shan Wong, and Martin D. Brand10. Imaging Mitochondrial Calcium Fluxes with Fluorescent Probes and Single or Two Photon Confocal MicroscopySean M. Davidson and Michael R. Duchen11. Mitochondrial Permeability Transition Pore and Calcium HandlingRandi J. Parks, Elizabeth Murphy, and Julia C. Liu12. Redox Equivalents and Mitochondrial BioenergeticsJames R. Roede, Young-Mi Go, and Dean P. Jones13. Mitochondrial Bioenergetics by 13 C-NMR Isotopomer AnalysisRui A. Carvalho14. Computational Modeling of Mitochondrial Function from a Systems Biology PerspectiveSonia Cortassa, Steven J. Sollott, and Miguel A. Aon15. Monitoring the Mitochondrial Dynamics in Mammalian CellsLuca Simula and Silvia Campello16. Plate-Based Measurement of Superoxide and Hydrogen Peroxide Production by Isolated MitochondriaHoi-Shan Wong, Pierre-Axel Monternier, Adam L. Orr, and Martin D. Brand17. Plate-Based Measurement of Respiration by Isolated MitochondriaShona A. Mookerjee, Casey L. Quinlan, Helen Wong, Pratiksha Dighe, and Martin D. Brand18. Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian CellsShun-ichi Yamashita and Tomotake Kanki19. The Importance of Calcium Ions for Determining Mitochondrial Glycerol-3-Phosphate Dehydrogenase Activity When Measuring Uncoupling Protein 1 (UCP1) Function in Mitochondria Isolated from Brown Adipose TissueKieran J. Clarke and Richard K. Porter20. Isolation and Analysis of Mitochondrial Small RNAs from Rat Liver Tissue and HepG2 CellsJulian Geiger and Louise T. Dalgaard21. Imaging of Mitochondrial pH Using SNARF-1Venkat K. Ramshesh and John J. Lemasters22. Relation between Mitochondrial Membrane Potential and ROS FormationJan Suski, Magdalena Lebiedzinska, Massimo Bonora, Paolo Pinton, Jerzy Duszynski, and Mariusz R. Wieckowski23. Assessing Spatio-Temporal and Functional Organization of Mitochondrial NetworksFelix T. Kurz, Miguel A. Aon, Brian O'Rourke and Antonis A. Armoundas24. Measurement of Mitochondrial ROS FormationSoni Deshwal, Salvatore Antonucci, Nina Kaludercic, and Fabio Di Lisa